Key result
Oral administration of PSH-3kDa fraction or peptide IYSPH caused a significant systolic blood pressure reduction of -30 mmHg in spontaneously hypertensive rats after 3-6 hours of treatment.
Why the study?
A peach seed hydrolysate peptide fraction below 3 kDa previously demonstrated high ACE inhibitory activity, prompting further isolation, characterization, and in vivo assessment of its antihypertensive capacity.
Does oral administration of peach seed hydrolysate peptides (PSH-3kDa or IYSPH) reduce systolic blood pressure in spontaneously hypertensive rats?
Does oral administration of peach seed hydrolysate peptides (PSH-3kDa or IYSPH) reduce systolic blood pressure in spontaneously hypertensive rats?
Mean Difference: -30
Peptides isolated from peach seed hydrolysates demonstrate significant ACE-inhibitory activity and effectively reduce systolic blood pressure in spontaneously hypertensive rats, suggesting potential utility as functional food ingredients.
Hypothesis-generating for peach seed peptides in hypertension; animal data leave human translation and practice change open.
A peptide fraction with molecular masses below 3 kDa (PSH-3 kDa) from a peach seed hydrolysate demonstrated high angiotensin converting enzyme (ACE) inhibitory activity (concentration to inhibit 50% ACE (IC 50 ) = 16.4 μg/mL) in our previous work. This work proposes a further study of this highly active fraction. RP-HPLC enabled two fractions (F3 and F4) with high inhibitory activity (IC 50 = 2.0 ± 0.5 and 1.2 ± 0.2 μg/mL, respectively) to be isolated. Peptide analysis by LC-Q-TOF-MS/MS using reverse-phase and hydrophilic interaction chromatography enabled 33 peptides within both fractions to be identified. Among them, peptide isoleucine–tyrosine–serine–proline–histidine (IYSPH) showed the highest capacity. The lack of cytotoxicity of peptides was demonstrated in three different cell lines (HeLa, HT-29, and HK-2). Oral administration of PSH-3 kDa fraction or peptide IYSPH caused a significant systolic blood pressure reduction (−30 mmHg) on spontaneously hypertensive rats after 3–6 h treatment.
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Vásquez-Villanueva et al. (2019) studied Hypertension (n=6). PSH-3kDa fraction or peptide IYSPH vs. Tap water (negative control) and captopril (10 mg/kg, positive control) was evaluated on Systolic blood pressure reduction (-30 mmHg). Oral administration of PSH-3kDa fraction or peptide IYSPH caused a significant systolic blood pressure reduction of -30 mmHg in spontaneously hypertensive rats after 3-6 hours of treatment.
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