Key result
Myofiber disarray quantified by image analysis was significantly greater in hypertrophic cardiomyopathy than other groups and correlated with IVST/LVPWT (r=0.70) and LVEDP (r=0.66, p<0.05).
Why the study?
Can an image analyzer effectively quantify myofiber disorientation in endomyocardial biopsy specimens and correlate with clinical findings in hypertrophic cardiomyopathy?
Cross-Sectional
Can an image analyzer effectively quantify myofiber disorientation in endomyocardial biopsy specimens and correlate with clinical findings in hypertrophic cardiomyopathy?
Effect estimate: r = 0.70
p-value: p=<0.05
An image analyzer provides a simple and useful method for quantifying myofiber disorientation in endomyocardial biopsies, which correlates significantly with clinical severity markers in hypertrophic cardiomyopathy.
May aid objective biopsy assessment in HCM; hypothesis-generating and should not yet change practice.
The disarray of cardiac myofibers was morphometrically evaluated in biopsy specimens, obtained from patients with hypertrophic cardiomyopathy (HCM), hypertensive heart disease (HHD), chronic phase myocarditis and controls. Microphotographs of myocardium were taken at a final magnification of x 250. For each segment of myofibers, the longitudinal direction was traced on a transparent sheet, and the angle of the traced direction to the baseline was measured by an image analyzer. The standard deviation of the angles was used as an indicator of myofiber disarray. The histograms showed a narrow variation in the control group, but a wide variation in the patients with HCM, HHD and chronic phase myocarditis. The mean value of standard deviations of myofiber angles in HCM was significantly larger than that in the other groups. In HCM, the standard deviation of the myofiber angle proved to correlate positively with IVST/LVPWT (the thickness ratio of interventricular septum to the left ventricular posterior wall) (r = 0.70, p < 0.05), and also with LVEDP (left ventricular end-diastolic pressure) (r = 0.66, p < 0.05). In conclusion, the image analyzer serves as a simple and useful tool in quantifying the disorientation of myofibers and estimating the correlation between the histological and clinical findings.
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Masuda et al. (1996) conducted a cross-sectional in Hypertrophic cardiomyopathy, hypertensive heart disease, chronic phase myocarditis. Hypertrophic cardiomyopathy vs. Controls, hypertensive heart disease, and chronic phase myocarditis was evaluated on Standard deviation of myofiber angles (r = 0.70, p=<0.05). Myofiber disarray quantified by image analysis was significantly greater in hypertrophic cardiomyopathy than other groups and correlated with IVST/LVPWT (r=0.70) and LVEDP (r=0.66, p<0.05).
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