Revealing the anti-ferroptosis guanidine alkaloids from Buthus martensii Karsch via a NMR-guided isolation combined with in vitro, in silico, and network pharmacology

Q: What does this research mean for the field?

Guanidine alkaloids isolated from Buthus martensii Karsch inhibit erastin-induced ferroptosis in cardiomyocytes by modulating core targets including AKT1 and the PI3K-Akt signaling pathway. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

Wen-Kai Zhao; Tao-Li Hao; Ya-Zhou Kuang; Tong Sun; Hongbing Zhang; Yu‐Ming Liu

doi:10.1080/14786419.2026.2725781

September 2, 2026Natural Product Research

Revealing the anti-ferroptosis guanidine alkaloids from Buthus martensii Karsch via a NMR-guided isolation combined with in vitro, in silico, and network pharmacology

View at Publisher

Ask AI

Bookmark

Share

Key Points

Identify anti-ferroptotic guanidine alkaloids from Buthus martensii Karsch and elucidate their protective molecular mechanisms in cardiac cells.
Purified guanidine alkaloids from Buthus martensii Karsch using an NMR-guided isolation approach.
Evaluated cytoprotective effects against erastin-induced ferroptotic cell death in H9c2 cardiomyocytes in vitro.
Characterized molecular interactions and targets through network pharmacology and computational molecular docking.
Guanidine alkaloids significantly attenuated erastin-induced ferroptosis in cultured H9c2 cardiomyocytes.
Network pharmacology implicated modulation of core ferroptotic targets including AKT1, EGFR, SRC, HSP90AA1, CASP3, and ESR1, primarily engaging the PI3K-Akt signaling pathway.
Molecular docking demonstrated strong binding affinities and structural stabilities between the isolated alkaloids and their predicted target proteins.

Ask about this paper

Answers are grounded in this paper and cite their sources.

Implication

In vitro study reveals guanidine alkaloids inhibit ferroptosis in H9c2 cardiomyocytes, suggesting potential therapeutic strategies against cardiac injury.

Abstract

significantly inhibited erastin-induced ferroptosis in H9c2 cardiomyocytes. Through network pharmacology, these alkaloids appeared to exert their effects by modulating core targets related to ferroptosis, such as AKT1, EGFR, SRC, HSP90AA1, CASP3, and ESR1, with the notable impacts on the PI3K-Akt signalling pathway and Lipid and atherosclerosis. Molecular docking analysis confirmed the strong binding affinities and stabilities between guanidine alkaloids and these targets.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Zhao et al. (2026) studied this question.

synapsesocial.com/papers/6a9bd3c76b95aff0620eaf0b https://doi.org/10.1080/14786419.2026.2725781

View at Publisher

Ask AI

Bookmark

Share

Revealing the anti-ferroptosis guanidine alkaloids from Buthus martensii Karsch via a NMR-guided isolation combined with in vitro, in silico, and network pharmacology

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Revealing the anti-ferroptosis guanidine alkaloids from Buthus martensii Karsch via a NMR-guided isolation combined with in vitro, in silico, and network pharmacology

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Keep reading

Keep reading