Biofouling by Limnoperna fortunei in Water-Conveyance Infrastructure: Stage-Specific Risks, Monitoring Signals, and Integrated Management for Sustainable Operation

The planktonic dispersal of Limnoperna fortunei larvae and the byssal attachment of juveniles and adults make this species a major invasive biofouling species in water-conveyance infrastructure, while artificial hydraulic connectivity further facilitates its spread. Dense colonization can reduce conveyance capacity, increase energy consumption, accelerate structural deterioration, and impair water quality, thereby posing multiple risks to infrastructure operation. This narrative and critical review synthesizes evidence from 110 publications retained after screening 537 records retrieved from the Web of Science Core Collection up to 30 June 2026. The review characterizes the stage-specific progression of L. fortunei biofouling from propagule input and early settlement to mature fouling and post-treatment residual risks. It compares the applicability of eDNA/qPCR assays, conventional field surveys, and remotely operated vehicle (ROV)-based image inspection, and evaluates the effectiveness and operational limitations of physical, chemical, coating-based, and biological control measures across different risk stages. Current management often targets individual stages, with limited linkage between monitoring results and subsequent intervention. Accordingly, we propose a risk-oriented decision pathway that integrates early warning, settlement confirmation, fouling-load assessment, targeted removal, and post-treatment verification while accounting for hydraulic safety, water-quality constraints, and asset accessibility. By aligning management actions with biofouling stage and asset condition, this framework provides a basis for more sustainable operation and maintenance of water-conveyance systems.