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September 5, 2026BMC Cardiovascular DisordersOpen Access

Serum miR-744-5p enables diagnosis of acute coronary syndrome and regulates inflammation and endothelial injury

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Key result

Downregulated serum miR-744-5p identifies ACS with a diagnostic AUC of ~0.91.

  • AUC 0.9103
  • 95% CI 0.8579-0.9627
  • P<0.0001
  • n=159

Why the study?

ACS presents a high risk of missed detection and misdiagnosis in early clinical diagnosis, and aberrant miR-744-5p expression has been observed in CAD.

Population

86 patients diagnosed with ACS and 73 control individuals

Comparison

Patients diagnosed with ACS vs control individuals

Design

Case-control and in vitro study

Authors

WWWenhua WangZhengzhou Central HospitalRLRongwen LinLongyan UniversityCZChangpeng ZuoXuzhou Medical College

Discussion

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Member takes

Implication

Supports miR-744-5p as potential ACS biomarker; extends prior observations but leaves open clinical adoption pending prospective validation.

Key Points

  • To assess the diagnostic value of circulating serum miR-744-5p in acute coronary syndrome (ACS) and characterize its regulatory mechanism on endothelial damage and inflammation through targeting filamin A (FLNA).
  • Quantified serum miR-744-5p levels via RT-qPCR in 86 patients diagnosed with ACS and 73 control individuals to perform ROC curve and multivariate logistic regression analyses.
  • Cultured human aortic endothelial cells subjected to 12-hour hypoxia/reoxygenation (H/R) injury with or without miR-744-5p mimic transfection to assess cell viability, inflammatory cytokines, and injury markers.
  • Identified and verified direct binding between miR-744-5p and FLNA using bioinformatic prediction, dual-luciferase reporter assays, and RNA immunoprecipitation.
  • Serum miR-744-5p expression was significantly downregulated in ACS patients compared with controls (P < 0.0001), distinguishing ACS with an AUC of 0.9103 (95% CI: 0.8579–0.9627), sensitivity of 89.53%, and specificity of 87.67%.
  • Multivariate logistic regression revealed that miR-744-5p is an independent protective factor against ACS (OR = 0.023, 95% CI: 0.008–0.066, P < 0.0001), while hypertension is an independent risk factor (OR = 3.225, 95% CI: 1.207–8.616, P = 0.020).
  • In H/R-treated endothelial cells, miR-744-5p directly targeted FLNA, restoring cell proliferative capacity (P < 0.001) and significantly reducing levels of vWF, H-FABP, IL-1β, IL-6, and TNF-α (P < 0.01).

Study Design

Type

Case-Control (n=159)

Multicenter

No

Structured PICO

P
Population
159 participants, comprising 86 patients with acute coronary syndrome and 73 healthy controls, were evaluated to determine the diagnostic efficacy of serum miR-744-5p.
O
Outcome
Diagnostic efficacy of serum miR-744-5p for acute coronary syndrome (measured by Area Under the Receiver Operating Characteristic Curve)surrogate

Main Result

Effect estimate: AUC 0.9103 (95% CI 0.8579-0.9627)

p-value: p=<0.0001

Serum miR-744-5p is significantly downregulated in acute coronary syndrome, demonstrating high diagnostic potential while mechanistically attenuating endothelial injury and inflammation by targeting FLNA.

Limitations

  • Specific downstream signaling pathways of FLNA remain to be fully elucidated.
  • Function of miR-744-5p within cardiomyocytes and its direct regulatory effect on cardiac function need further exploration.
  • Relatively small sample size prevents subgroup analysis of miR-744-5p expression across different ACS subtypes.
  • Findings are mainly derived from in vitro experiments and overexpression assays, requiring further in vivo validation.
  • The endogenous reference gene adopted for miRNA normalization exhibits lower stability than alternative endogenous references.

Cite This Study

Wang et al. (2026) conducted a case-control in Acute coronary syndrome (n=159). Serum miR-744-5p vs. Healthy controls was evaluated on Diagnostic performance for acute coronary syndrome (AUC 0.9103, 95% CI 0.8579-0.9627, p=<0.0001). Serum miR-744-5p was significantly downregulated in patients with acute coronary syndrome, demonstrating high diagnostic efficacy with an AUC of 0.9103.

synapsesocial.com/papers/6a9bd48c6b95aff0620ec388https://doi.org/10.1186/s12872-026-06412-5
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