Key result
Riboflavin photo-cross-linked pericardiums demonstrated improved collagen and elastin stability, better resistance to calcification, and fewer CD68 positive macrophages in a rat implantation model.
Why the study?
Glutaraldehyde cross-linked bioprosthetic heart valves can fail due to progressive degradation and calcification.
A novel riboflavin photo-cross-linking strategy for bioprosthetic heart valves improves elastin stability, reduces calcification, and decreases inflammatory response in preclinical models.
May advance bioprosthetic durability; leaves open translation from rat models to clinical xenotransplantation.
BACKGROUND: Glutaraldehyde cross-linked bioprosthetic heart valves might fail due to progressive degradation and calcification. METHODS: In this study, we developed a new BHVs preparation strategy named as "HPA/TRA/FMN" that utilized 3,4-hydroxyphenylpropionic acid (HPA)/tyramine (TRA) conjugated pericardium and riboflavin 5'-monophosphate (FMN) initiated photo-cross-linking method. HPA/TRA-pericardium conjugation would provide extra phenol groups for FMN initiated photo-cross-linking. RESULTS: The feeding ratio of riboflavin 5'-monophosphate was optimized. The collagenase and elastase enzymatic degradation in vitro, biomechanics, calcification, elastin stability in vivo, and macrophage marker CD68 were characterized. We demonstrated that riboflavin photo-cross-linked pericardiums had great collagen and elastin stability, improved mechanical properties, better resistance for calcification, and less CD68 positive macrophages in rat subdermal implantation study. CONCLUSIONS: This new riboflavin photo-cross-linking strategy would be a promising method to make BHVs which have better elastin stability, less calcification, and reduced inflammatory response.
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Yang et al. (2019) studied Bioprosthetic heart valve degradation and calcification. Riboflavin photo-cross-linking method (HPA/TRA/FMN) was evaluated on Collagen and elastin stability, biomechanics, calcification, and macrophage marker CD68. Riboflavin photo-cross-linked pericardiums demonstrated improved collagen and elastin stability, better resistance to calcification, and fewer CD68 positive macrophages in a rat implantation model.
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