Key result
HPA/NT/HRP treatment improves glycosaminoglycan stability and reduces calcification in bioprosthetic valve pericardium versus GLUT.
Why the study?
Glutaraldehyde-crosslinked bioprosthetic heart valves often fail due to degradation and calcification, partly because glutaraldehyde cannot stabilize glycosaminoglycans.
Does HPA/NT/HRP enzyme-oxidative-polymerization improve glycosaminoglycan stability and reduce calcification in bioprosthetic heart valve pericardium tissue?
Does HPA/NT/HRP enzyme-oxidative-polymerization improve glycosaminoglycan stability and reduce calcification in bioprosthetic heart valve pericardium tissue?
A novel HPA/NT/HRP enzyme-oxidative-polymerization strategy improves glycosaminoglycan stability and reduces calcification in bioprosthetic heart valve tissues in preclinical models.
May reduce bioprosthetic valve calcification; leaves open clinical translation from this animal model.
Glutaraldehyde (GLUT) crosslinked bioprosthetic heart valves (BHVs) might fail within ten years due to progressive degradation and calcification. GLUT cannot stabilize glycosaminoglycans (GAGs), one important component of BHVs. In this current study we developed a new BHVs preparation strategy named as ‘HPA/NT/HRP’ treatment that utilized 3,4-hydroxyphenylpropionic acid (HPA)/tyramine (TRA)/neomycin trisulfate (NT) conjugated pericardiums and horseradish peroxidase (HRP)/H 2 O 2 enzyme-oxidative-polymerization method. HPA/TRA-pericardium and HPA-NT conjugation would provide extra phenol groups for enzymatic crosslinking. HPA/TRA conjugated pericardium could be crosslinked by HRP/H 2 O 2 enzyme-oxidative-polymerization. The feeding ratio of HPA-NT was optimized. The GAGs content, collagenase and elastase degradation in vitro , the in vivo GAGs stability and anti-calcification potential of HPA/NT/HRP treated pericardiums were characterized. We demonstrated that HPA/NT/HRP treated pericardiums had sufficiently increased GAGs stabilization and decreased calcification. This new HPA/NT/HRP enzyme-oxidative-polymerization strategy would be a promising method to make BHVs with better GAGs stability and anti-calcification properties.
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Yang et al. (2019) studied Bioprosthetic heart valve degradation and calcification. HPA/NT/HRP enzyme-oxidative-polymerization treatment vs. Glutaraldehyde (GLUT) crosslinking was evaluated on Glycosaminoglycans (GAGs) stability and anti-calcification potential. HPA/NT/HRP treated pericardiums demonstrated increased glycosaminoglycan stabilization and decreased calcification, offering a promising strategy for bioprosthetic heart valves.
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