Key result
GPTMS crosslinked porcine pericardium showed no cytotoxicity, supported endothelial cell growth, had comparable mechanical properties to Glut-PP, and effectively inhibited calcification in rats.
Why the study?
Commercially used glutaraldehyde-treated bioprosthetic heart valves often fail within 12-15 years due to degradation, thrombosis, inferior biocompatibility, and calcification.
Does GPTMS crosslinking improve the biocompatibility, mechanical properties, and calcification resistance of porcine pericardium for bioprosthetic heart valves compared to glutaraldehyde?
Does GPTMS crosslinking improve the biocompatibility, mechanical properties, and calcification resistance of porcine pericardium for bioprosthetic heart valves compared to glutaraldehyde?
GPTMS crosslinking of porcine pericardium improves biocompatibility, anti-tearing performance, and calcification resistance compared to standard glutaraldehyde treatment, suggesting potential as a superior biomaterial for bioprosthetic heart valves.
GPTMS crosslinking of PP merits further testing as a Glut alternative; leaves open durability, thrombosis, and calcification outcomes in vivo.
Bioprosthetic heart valve (BHV) replacement is increasingly used for treating valve-related diseases worldwide but the current commercially used BHVs treated with glutaraldehyde (Glut) often failed within 12-15 years due to degradation, thrombosis, inferior biocompatibility, and calcification. Herein, 3-glycidyloxypropyl trimethoxysilane (GPTMS) was used to crosslink porcine pericardium (PP) at the concentration (vol/vol) of 0.25%, 1%, 2%, and 4% and their performance for potential application in BHVs was evaluated. The crosslinking mechanism mainly involved the ring-opening of epoxide by amine attack and silanol poly-condensation. The stability of collagen in higher concentration (1%, 2%, and 4%) GPTMS crosslinked PPs (GPTMS-PPs) was clearly increased. GPTMS-PPs showed no cytotoxicity and supported the growth of endothelial cells while Glut-PP did not. GPTMS-PPs were less prothrombotic than Glut-PP. GPTMS-PP crosslinked at 1% concentration showed comparable mechanical properties to Glut-PP while had better anti-tearing performance. The subcutaneous implantation in rat for 30 days showed that GPTMS crosslinking was able to effectively inhibit the calcification of BHV.
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Yang et al. (2020) studied Valve-related diseases. 3-glycidyloxypropyl trimethoxysilane (GPTMS) crosslinked porcine pericardium vs. Glutaraldehyde (Glut) crosslinked porcine pericardium was evaluated on Cytotoxicity, endothelial cell growth, prothrombotic activity, mechanical properties, and calcification. GPTMS crosslinked porcine pericardium showed no cytotoxicity, supported endothelial cell growth, had comparable mechanical properties to Glut-PP, and effectively inhibited calcification in rats.
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