Key result
Cross-linking porcine pericardium with DHBA compared to GLUT decreased relative platelet absorption (0.176 vs 0.294) and increased APTT (15.2 vs 9.9 s), indicating improved hemocompatibility.
Why the study?
Clinically applied glutaraldehyde-cross-linked bioprosthetic heart valves have drawbacks including high cytotoxicity, severe calcification, and poor hemocompatibility.
Does cross-linking with 3,4-dihydroxybenzaldehyde (DHBA) improve the biocompatibility of porcine pericardium compared to glutaraldehyde?
Does cross-linking with 3,4-dihydroxybenzaldehyde (DHBA) improve the biocompatibility of porcine pericardium compared to glutaraldehyde?
DHBA cross-linking of porcine pericardium improves hemocompatibility, anticalcification, and cytocompatibility compared to standard glutaraldehyde cross-linking in a preclinical model.
DHBA cross-linking may improve valve materials; hypothesis-generating and should not yet change clinical practice.
Heart valve replacement is an effective therapy for patients with moderate to severe valvular stenosis or regurgitation. Most bioprosthetic heart valves applied clinically are based on cross-linking with glutaraldehyde (GLUT), but they have some drawbacks like high cytotoxicity, severe calcification, and poor hemocompatibility. In this study, we focused on enhancing the properties of bioprosthetic heart valves by cross-linking with 3,4-dihydroxybenzaldehyde (DHBA). The experiment results revealed that compared with GLUT cross-linked porcine pericardium (PP), the relative amount of platelets absorbed on the surface of DHBA cross-linked PP decreased from 0.294 ± 0.034 to 0.176 ± 0.028, and the activated partial thromboplastin time (APTT) increased from 9.9 ± 0.1 to 15.2 ± 0.1 s, indicating improved hemocompatibility. Moreover, anticalcification performance and cytocompatibility were greatly enhanced by DHBA cross-linking. In conclusion, the properties of bioprosthetic valves could be effectively improved by processing valves with a DHBA-based cross-linking method.
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Wu et al. (2020) studied Valvular stenosis or regurgitation. 3,4-dihydroxybenzaldehyde (DHBA) cross-linking vs. Glutaraldehyde (GLUT) cross-linking was evaluated on Relative amount of platelets absorbed and activated partial thromboplastin time (APTT). Cross-linking porcine pericardium with DHBA compared to GLUT decreased relative platelet absorption (0.176 vs 0.294) and increased APTT (15.2 vs 9.9 s), indicating improved hemocompatibility.
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