Key result
Hyaluronic acid-grafted porcine pericardium significantly reduced calcium content compared to glutaraldehyde-crosslinked pericardium (37 ± 29 μg/mg vs 188 ± 7 μg/mg) in a rat subcutaneous model.
Why the study?
Bioprosthetic heart valves have problems with calcification, thrombogenicity, and cytotoxicity that glutaraldehyde-based crosslinking techniques struggle to resolve simultaneously.
Does hyaluronic acid grafting by copolymerization reduce calcification and thrombogenicity in glutaraldehyde-crosslinked porcine pericardium in a rat model?
Does hyaluronic acid grafting by copolymerization reduce calcification and thrombogenicity in glutaraldehyde-crosslinked porcine pericardium in a rat model?
Absolute Event Rate: 37% vs 188%
Grafting porcine pericardium with hyaluronic acid via copolymerization significantly reduces calcification and platelet adhesion in a rat model, suggesting potential for improving bioprosthetic heart valves.
Does not support clinical use of HA-grafted pericardium; leaves open durability benefits in human bioprostheses.
Bioprosthetic heart valves (BHVs) are widely used in clinic, but they still have problems of calcification, thrombogenicity, and cytotoxicity. The reported techniques based on glutaraldehyde (Glut) crosslinking have difficulty in solving these problems simultaneously. In this study, we grafted Glut-crosslinked porcine pericardium (GA) with hyaluronic acid (HA) by radical copolymerization to improve its anticalcification and antithrombotic properties. Partially methacrylated poly-ε-lysine was used to introduce methacryl groups into GA. Then, HA-grafted porcine pericardium (GA-HA) was obtained by radical copolymerization. Rat's subcutaneous implantation results showed that the calcium content of GA-HA was significantly lower than that of GA (37 ± 29 μg/mg vs 188 ± 7 μg/mg), and the platelets adhering to the surface of GA-HA decreased by approximately 41% compared with GA. In conclusion, grafting porcine pericardium with HA by copolymerization might be feasible to improve the anticalcification and antithrombotic properties of BHVs.
No takes yet. Share an insight, caveat, or question.
Huang et al. (2022) studied Bioprosthetic heart valve calcification and thrombogenicity. Hyaluronic acid-grafted porcine pericardium (GA-HA) vs. Glutaraldehyde-crosslinked porcine pericardium (GA) was evaluated on Calcium content after subcutaneous implantation. Hyaluronic acid-grafted porcine pericardium significantly reduced calcium content compared to glutaraldehyde-crosslinked pericardium (37 ± 29 μg/mg vs 188 ± 7 μg/mg) in a rat subcutaneous model.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: