Key result
A hybrid pericardium with VEGF-loaded hyaluronic acid hydrogel coating improved endothelial cell adhesion and proliferation, and reduced platelet adhesion and calcification in preclinical models.
Why the study?
Bioprosthetic heart valves are mostly fixed by glutaraldehyde, and the lack of endothelialization is a major problem for glutaraldehyde-fixed pericardia.
Coating glutaraldehyde-fixed pericardium with a VEGF-loaded hyaluronic acid hydrogel improves endothelialization and reduces calcification and thrombosis in preclinical models, suggesting potential for better bioprosthetic heart valves.
Hypothesis-generating for coated bioprostheses; should not change practice until human trials confirm translation.
Bioprosthetic heart valves (BHVs) used in the clinic are mostly fixed by glutaraldehyde and the lack of endothelialization is a major problem for glutaraldehyde-fixed pericardia. Hyaluronic acid is a major glycosaminoglycan that exists in native heart valves. Coupled with its inherent biocompatibility, it may enhance endothelial adhesion and proliferation when associated with vascular endothelial growth factor (VEGF). In this study, an optimized system is developed to improve the endothelialization of glutaraldehyde-fixed pericardium. A hybrid pericardium with VEGF-loaded hyaluronic acid hydrogel coating is developed by the crosslinking of 1,4-butanediol diglycidyl ether. The adhesion and growth potential of human umbilical vein endothelial cells (HUVECs) on pericardia, platelet adhesion, and calcification by an in vivo rat subdermal implantation model are investigated. The results show improved HUVEC adhesion and proliferation, less platelet adhesion, and less calcification for hybrid pericardium by introducing the coating of VEGF-loaded hyaluronic acid hydrogel. Thus, the coating of VEGF-loaded hyaluronic acid hydrogel on pericardium is a promising approach to obtain bioprosthetic valves for clinical applications with increased endothelialization and antithrombotic and anticalcification properties.
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Yang et al. (2019) studied Bioprosthetic heart valves. Hybrid pericardium with VEGF-loaded hyaluronic acid hydrogel coating vs. Glutaraldehyde-fixed pericardium was evaluated on HUVEC adhesion and proliferation, platelet adhesion, and calcification. A hybrid pericardium with VEGF-loaded hyaluronic acid hydrogel coating improved endothelial cell adhesion and proliferation, and reduced platelet adhesion and calcification in preclinical models.
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