Key result
The branched fluorescent peptide probe 2PF demonstrated a 16-fold increase in fluorescence intensity compared to clot autofluorescence and a 5.5-fold increase compared to the control probe.
Why the study?
Does the branched fluorescent peptide probe 2PF improve detection of activated platelets in clots compared to linear or control probes?
Does the branched fluorescent peptide probe 2PF improve detection of activated platelets in clots compared to linear or control probes?
A novel branched fluorescent peptide probe (2PF) targeting GP-IIb/IIIa demonstrates high binding affinity to activated platelets, suggesting potential utility for in vivo thrombosis imaging.
Answers are grounded in this paper and cite their sources.
May advance targeted platelet imaging; leaves open human translation and clinical validation.
Novel fluorescent probes for thrombi and activated-platelet detection were developed that were based on the glycoprotein IIb/IIIa (GP-IIb/IIIa) binding sequence, Pro-Ser-Pro-Gly-Asp-Trp. Linear, Pro-Ser-Pro-Gly-Asp-Trp-Aha-Gly-Cys(Cy5.5)-NH(2) (1PF), and branched, (Pro-Ser-Pro-Gly-Asp-Trp-Aha)(2)-Lys-Gly-Cys(Cy5.5)-NH(2) (2PF), fluorescent-labeled peptide probes were synthesized. A third probe, also branched, (Pro-Ser-Pro-Gly-Glu-Trp-Aha)(2)-Lys-Gly-Cys(Cy5.5)-NH(2) (2CF), was synthesized as control. The platelet-binding activity of the probes was tested in clots generated from human platelet-rich plasma. Fluorescence reflectance imaging results showed that 2PF has a 16-fold increase in fluorescence intensity compared to the autofluorescence of clots. The linear conjugate, 1PF, and free dye did not show appreciable fluorescence enhancement. 2PF fluorescence was also found 5.5-fold higher than that of the control probe, 2CF. Overall, our results suggest that 2PF binds tightly to GP-IIb/IIIa and potentially can be used for in vivo imaging of thrombosis.
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Tung et al. (2004) studied Thrombosis. Branched fluorescent-labeled peptide probe (2PF) vs. Linear conjugate (1PF), free dye, and control probe (2CF) was evaluated on Fluorescence intensity in clots (16-fold increase vs autofluorescence). The branched fluorescent peptide probe 2PF demonstrated a 16-fold increase in fluorescence intensity compared to clot autofluorescence and a 5.5-fold increase compared to the control probe.
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