Key result
In rabbit proximal convoluted tubules, basolateral chloride conductance is negligible, while SITS-insensitive KCl cotransport and SITS-sensitive Na-dependent Cl-HCO3 exchange are present.
Population
Rabbit proximal convoluted tubules (PCT) perfused in vitro
Comparison
Various bath conditions including voltage clamp… vs Baseline/control bath conditions
Design
Preclinical
Authors
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Challenges conductance-centric models of proximal Cl− transport; leaves open human relevance and clinical translation.
The study identifies that basolateral chloride transport in rabbit proximal convoluted tubules is mediated by SITS-insensitive KCl cotransport and SITS-sensitive Na-dependent Cl-HCO3 exchange, rather than simple chloride conductance.
Ishibashi et al. (1990) studied this question. Bath ion substitutions and inhibitors (SITS, barium, furosemide) vs. Baseline/spontaneous conditions was evaluated on Intracellular Cl activity (AiCl) and basolateral membrane voltage (Vbl). In rabbit proximal convoluted tubules, basolateral chloride conductance is negligible, while SITS-insensitive KCl cotransport and SITS-sensitive Na-dependent Cl-HCO3 exchange are present.
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