Key result
An indirect ELISA for detecting duck hepatitis virus antibodies demonstrated a positive detection rate of 68.8%, matching the serum-neutralization test and exceeding the 18.8% rate of the AGDP test.
Why the study?
Is ELISA a sensitive and specific method for detecting antibodies to duck hepatitis virus compared to AGDP and SN tests?
Observational (n=93)
Is ELISA a sensitive and specific method for detecting antibodies to duck hepatitis virus compared to AGDP and SN tests?
ELISA is a rapid, sensitive, and accurate method for detecting antibodies to duck hepatitis virus, performing comparably to the SN test and better than the AGDP test.
May support ELISA adoption for duck hepatitis serology; leaves open prospective validation before routine use.
An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibody to duck hepatitis virus (DHV) is described. The results of ELISA were compared with those of an agar gel diffusion precipitin (AGDP) test and a serum-neutralization (SN) test. The specificity of the ELISA was in accordance with the specificity of the AGDP and SN tests, but there was a difference in sensitivity. The positive detection rates of ELISA, SN test, and AGDP test for 93 clinical samples were 68.8%, 68.8%, and 18.8%, respectively. A positive/negative (P/N) value larger than or equal to 2.1 plus an absorbance value larger than or equal to 0.4 was used as a comprehensive positive standard for the ELISA. This eliminated false-positive reactions. The results showed that the ELISA was a rapid, sensitive, and accurate method for detecting antibody to DHV.
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Phillips et al. (1991) conducted an observational in Duck hepatitis virus (DHV) antibody detection (n=93). Enzyme-linked immunosorbent assay (ELISA) vs. Agar gel diffusion precipitin (AGDP) test and serum-neutralization (SN) test was evaluated on Positive detection rate. An indirect ELISA for detecting duck hepatitis virus antibodies demonstrated a positive detection rate of 68.8%, matching the serum-neutralization test and exceeding the 18.8% rate of the AGDP test.
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