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July 1, 2004Australian Veterinary Journal

Implementation in Australia of molecular diagnostic techniques for the rapid detection of foot and mouth disease virus

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Key result

Both PCR TaqMan assays targeted to the FMDV IRES region and the 3D polymerase coding region successfully detected FMDV genetic material from multiple strains in non-infectious antigen stocks.

Population

Non-infectious FMDV ELISA antigen stocks held at Australian Animal Health Laboratory

Design

Preclinical

Authors

DBDB BOYLETTT V TaylorBaylor College of MedicineMCMaria Cecilia A Cardoso

Discussion

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Implication

Supports dual-target PCR for FMDV detection in antigen stocks; leaves open prospective validation in infectious field samples.

Structured PICO

P
Population
Evaluation of two PCR TaqMan assays using non-infectious FMDV ELISA antigen stocks held at the Australian Animal Health Laboratory.
I
Intervention
PCR TaqMan assays targeted to the FMDV internal ribosome entry site (IRES) or the 3D polymerase coding region
O
Outcome
Detection of FMDV genetic materialsurrogate

Both PCR TaqMan assays (IRES and 3D polymerase targets) are effective for rapid detection of FMDV and are implemented together in Australia for independent confirmation.

Limitations

  • The TaqMan assay directed to the FMDV IRES is the only one to date to have undergone substantial evaluation using clinical samples collected during an outbreak.

Cite This Study

BOYLE et al. (2004) studied Foot and mouth disease virus (FMDV). PCR TaqMan assays targeted to the FMDV IRES region or the 3D polymerase coding region was evaluated on Detection of FMDV genetic material. Both PCR TaqMan assays targeted to the FMDV IRES region and the 3D polymerase coding region successfully detected FMDV genetic material from multiple strains in non-infectious antigen stocks.

synapsesocial.com/papers/6a9c59399beff30feb5d2777https://doi.org/10.1111/j.1751-0813.2004.tb11133.x
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Also Consider

Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Detection of Antibodies against Foot-and-Mouth Disease Virus Using a Liquid-Phase Blocking Sandwich ELISA (LPBE) with a Bioengineered 3D Protein1996 · 32 citations
  2. 2The early pathogenesis of foot-and-mouth disease in pigs infected by contact: a quantitative time-course study using TaqMan RT–PCR2001 · 132 citations