What question did this study set out to answer?

Determine whether conditioned medium from cord or placental mesenchymal stem cells protects ex vivo expanded umbilical cord blood CD34+ cells against oxidative stress during cryopreservation.

October 25, 2016PLoS ONEOpen Access

Conditioned Medium from Placental Mesenchymal Stem Cells Reduces Oxidative Stress during the Cryopreservation of Ex Vivo Expanded Umbilical Cord Blood Cells

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Authors

DKDarshana KadekarAarhus University SRSonal Rangole VKVaijayanti KaleSymbiosis International University

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Implication

In vitro study reveals that placental mesenchymal stem cell conditioned medium reduces cryopreservation oxidative stress in expanded cord blood cells, indicating improved clinical utility.

Key Points

Determine whether conditioned medium from cord or placental mesenchymal stem cells protects ex vivo expanded umbilical cord blood CD34+ cells against oxidative stress during cryopreservation.
Expanded umbilical cord blood CD34+ cells for 10 days in cytokine-supplemented serum-free suspension cultures.
Cryopreserved expanded cells in conventional freezing medium (20% FCS + 10% DMSO) with or without cord- or placental-derived mesenchymal stem cell conditioned medium (C-MSCs-CM, P-MSCs-CM) using a programmable freezer.
Assessed post-thaw cell viability, CD34+ cell retention, clonogenicity, oxidative stress markers, and transplantation potential after a 48-hour priming period.
Cryopreservation with MSCs-CM significantly improved post-thaw viability, CD34+ cell retention, and clonogenicity compared to standard freezing medium alone.
P-MSCs-CM markedly lowered oxidative stress and reactive oxygen species generation compared to C-MSCs-CM, operating via anti-oxidative and anti-apoptotic pathways rather than exosome activity.
Priming revived cells with MSCs-CM for 48 hours substantially enhanced their functional transplantation capacity.

Cite This Study

Kadekar et al. (2016) studied this question.

synapsesocial.com/papers/6a9c5f2718ccda70ef76dc9b https://doi.org/10.1371/journal.pone.0165466

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Implication

In vitro study reveals that placental mesenchymal stem cell conditioned medium reduces cryopreservation oxidative stress in expanded cord blood cells, indicating improved clinical utility.

Conditioned Medium from Placental Mesenchymal Stem Cells Reduces Oxidative Stress during the Cryopreservation of Ex Vivo Expanded Umbilical Cord Blood Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Conditioned Medium from Placental Mesenchymal Stem Cells Reduces Oxidative Stress during the Cryopreservation of Ex Vivo Expanded Umbilical Cord Blood Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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