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September 25, 2009Arteriosclerosis Thrombosis and Vascular Biology

Dynamic Observation of Mechanically-Injured Mouse Femoral Artery Reveals an Antiinflammatory Effect of Renin Inhibitor

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Key result

Aliskiren significantly reduced the number of adherent leukocytes in injured mouse femoral arteries compared to PBS (18.4 vs 43.8 per 10^-2 mm^2, P<0.05).

Why the study?

Does aliskiren reduce leukocyte adhesion and vascular inflammation in mechanically-injured mouse femoral arteries and activated endothelial cells?

Population

C57BL/6 mice with perivascular cuff injury to the femoral artery, and human umbilical vein endothelial cells…

Comparison

Aliskiren administered via osmotic pump for 2… vs PBS; untreated or TNF-alpha-activated HUVECs…

Design

Preclinical

Follow-up

2 weeks of treatment, 24 hours post-injury observation

Authors

JIJun InoCKChiari KojimaMOMizuko Osaka

Discussion

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Member takes

Overview

Aliskiren may limit vascular inflammation after injury; hypothesis-generating and requires human studies before any clinical consideration.

Structured PICO

Does aliskiren reduce leukocyte adhesion and vascular inflammation in mechanically-injured mouse femoral arteries and activated endothelial cells?

P
Population
C57BL/6 mice (6-7 weeks of age) subjected to femoral artery perivascular cuff injury and treated with aliskiren or PBS for 2 weeks.
I
Intervention
Aliskiren (10 mg/kg/d) administered via osmotic pump for 2 weeks (in vivo); aliskiren treatment (in vitro).
C
Comparator
PBS (in vivo); untreated or TNF-alpha-activated HUVECs without aliskiren (in vitro).
O
Outcome
Number of adherent leukocytes 24 hours after injury (in vivo) and adhesion of THP-1 cells to TNF-alpha-activated HUVECs (in vitro).surrogate

Main Result

Absolute Event Rate: 18.4% vs 43.8%

p-value: p=<0.05

Aliskiren demonstrates anti-inflammatory effects in vascular injury models independent of blood pressure changes.

Cite This Study

Ino et al. (2009) studied Vascular inflammation. Aliskiren vs. PBS was evaluated on Number of adherent leukocytes 24 hours after injury (p=<0.05). Aliskiren significantly reduced the number of adherent leukocytes in injured mouse femoral arteries compared to PBS (18.4 vs 43.8 per 10^-2 mm^2, P<0.05).

synapsesocial.com/papers/6a9c8be86d79abe55ff4f23bhttps://doi.org/10.1161/atvbaha.108.182519
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Effects of Aliskiren on Blood Pressure, Albuminuria, and (Pro)Renin Receptor Expression in Diabetic TG(mRen-2)27 Rats2008 · 286 citations
  2. 2Angiotensin II Induces Vascular Cell Adhesion Molecule-1 Expression In Rat Vasculature1999 · 378 citations
  3. 3Increased Accumulation of Tissue ACE in Human Atherosclerotic Coronary Artery Disease1996 · 426 citations
  4. 4Pivotal role of the renin/prorenin receptor in angiotensin II production and cellular responses to renin2002 · 1,140 citations
  5. 5Increased Chymase-Dependent Angiotensin II Formation in Human Atherosclerotic Aorta1999 · 200 citations