What question did this study set out to answer?

To determine whether cytomegalovirus infection modulates adhesion molecule expression across human arterial and microvascular endothelial cells representing target sites of vascular allograft rejection.

December 1, 1999Transplantation

Cytomegalovirus-Mediated Modulation of Adhesion Molecule Expression by Human Arterial and Microvascular Endothelial Cells1

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Why the study?

Does CMV infection modulate adhesion molecule expression in human arterial and microvascular endothelial cells?

Population

Human coronary artery, aortic, umbilical artery, and microvascular endothelial cells (EC)

Comparison

Cytomegalovirus infection and/or treatment with… vs Mock infection

Design

Preclinical

Authors

DKDeborah A. KnightThe Ohio State University WWW. James WaldmanGdańsk Medical University DSDaniel D. SedmakThe Ohio State University

Discussion

Member takes

Implication

May affect allograft immunogenicity; hypothesis-generating for CMV's role in transplant outcomes, pending human validation.

Key Points

To determine whether cytomegalovirus infection modulates adhesion molecule expression across human arterial and microvascular endothelial cells representing target sites of vascular allograft rejection.
Infected human coronary artery, aortic, umbilical artery, and microvascular endothelial cell isolates with cytomegalovirus or mock control.
Stimulated infected and uninfected endothelial cultures with tumor necrosis factor-alpha.
Evaluated adhesion molecule surface expression using flow cytometric and immunohistochemical analyses.
Cytomegalovirus infection directly upregulated intercellular adhesion molecule-1 (ICAM-1) across all tested arterial and microvascular endothelial cells, without inducing E-selectin or vascular cell adhesion molecule-1 (VCAM-1).
Infected endothelial cells became refractory to normal tumor necrosis factor-alpha-mediated induction of both E-selectin and vascular cell adhesion molecule-1.

Structured PICO

Does CMV infection modulate adhesion molecule expression in human arterial and microvascular endothelial cells?

Population

Human coronary artery, aortic, umbilical artery, and microvascular endothelial cells (EC)

Intervention

Cytomegalovirus (CMV) infection and/or treatment with tumor necrosis factor-alpha

Comparator

Mock infection

Outcome

Expression of intercellular adhesion molecule-1 (ICAM-1), E-selectin, and vascular cell adhesion molecule-1 (VCAM-1) measured by flow cytometry and immunohistochemistrysurrogate

CMV infection alters adhesion molecule expression uniformly across endothelial cells of different vascular origins, potentially affecting allograft immunogenicity.

Cite This Study

Knight et al. (1999) studied this question.

synapsesocial.com/papers/6a9cbfa118ccda70ef76eba3 https://doi.org/10.1097/00007890-199912150-00030

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Cytomegalovirus-Mediated Modulation of Adhesion Molecule Expression by Human Arterial and Microvascular Endothelial Cells1

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Cytomegalovirus-Mediated Modulation of Adhesion Molecule Expression by Human Arterial and Microvascular Endothelial Cells1

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider