Key result
Ultrafiltration of human plasma isolated small HDL particles (MW 70,000) containing cholesterol, phospholipids, and apolipoproteins A-I and A-II, comprising 67.5% protein by mass.
The study successfully isolated and characterized two distinct subpopulations of low molecular weight HDL particles from human plasma.
Does not alter lipid management; leaves open functional roles of these low-MW HDL particles in reverse cholesterol transport.
Small particles of high density lipoproteins (HDL) were isolated from fresh, fasting human plasma and from the ultracentrifugally isolated high density lipoprotein fraction by means of ultrafiltration through membranes of molecular weight cutoff of 70,000. These particles were found to contain cholesterol, phospholipids, and apolipoproteins A-I and A-II; moreover, they floated at a density of 1.21 kg/l. They contained 67.5% of their mass as protein and the rest as lipid. Two populations of small HDL particles were identified: one containing apolipoprotein A-I alone [(A-I)HDL] and the other containing both apolipoproteins A-I and A-II [A-I + A-II)HDL]. The molar ratio of apoA-I to apoA-II in the latter subclass isolated from plasma or HDL was 1:1. The molecular weights of these subpopulations were determined by nondenaturing gradient polyacrylamide gel electrophoresis and found to be 70,000; 1.5% of the plasma apoA-I was recovered in the plasma ultrafiltrate.
No takes yet. Share an insight, caveat, or question.
R G Atmeh (1990) studied this question. Ultrafiltration through membranes of molecular weight cutoff of 70,000 was evaluated on Isolation and characterization of small HDL particles. Ultrafiltration of human plasma isolated small HDL particles (MW 70,000) containing cholesterol, phospholipids, and apolipoproteins A-I and A-II, comprising 67.5% protein by mass.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: