What question did this study set out to answer?

To characterize human high-density lipoprotein (HDL) subfractions across a spectrum of hydrated densities and evaluate the distribution of cholesterol, apolipoprotein A-I, and apolipoprotein A-II.

February 1, 1979Journal of Lipid ResearchOpen Access

Distribution of cholesterol and apolipoprotein A-I and A-II in human high density lipoprotein subfractions separated by CsCl equilibrium gradient centrifugation: evidence for HDL subpopulations with differing A-I/A-II molar ratios.

View Full Paper

Ask AI

Bookmark

Authors

MCMC CheungHong Kong Polytechnic University JAJ J AlbersUnited States Geological Survey

Discussion

Member takes

Implication

Experimental study demonstrates varying apolipoprotein A-I to A-II molar ratios across density fractions in human serum, indicating distinct high-density lipoprotein subpopulations.

Key Points

To characterize human high-density lipoprotein (HDL) subfractions across a spectrum of hydrated densities and evaluate the distribution of cholesterol, apolipoprotein A-I, and apolipoprotein A-II.
Subfractionated whole serum and isolated HDL (density 1.063–1.25 g/ml) from three men and three women using cesium chloride equilibrium gradient centrifugation.
Quantified apolipoprotein A-I and A-II levels using radial immunodiffusion and determined apparent molecular weights across fractions via gradient gel electrophoresis.
The apolipoprotein A-I/A-II molar ratio remained constant at 2.2 ± 0.2 across densities 1.106–1.150 g/ml, increased with density from 1.151–1.25 g/ml, and increased with decreasing density between 1.077–1.105 g/ml (from 2.8 ± 0.5 at 1.093–1.105 g/ml to 5.6 ± 1.3 at 1.077–1.082 g/ml).
The cholesterol/(A-I + A-II) ratio and apparent molecular weight consistently decreased as hydrated density increased from 1.063 to 1.200 g/ml (molecular weight falling from 3.9 × 10⁵ to 1.1 × 10⁵) in both men and women.

Cite This Study

Cheung et al. (1979) studied this question.

synapsesocial.com/papers/6a82c36a3e02825336d119d2 https://doi.org/10.1016/s0022-2275(20)40631-5

View Full Paper

Ask AI

Bookmark

Implication

Experimental study demonstrates varying apolipoprotein A-I to A-II molar ratios across density fractions in human serum, indicating distinct high-density lipoprotein subpopulations.

Distribution of cholesterol and apolipoprotein A-I and A-II in human high density lipoprotein subfractions separated by CsCl equilibrium gradient centrifugation: evidence for HDL subpopulations with differing A-I/A-II molar ratios.

Discussion

Member takes

Implication

Key Points

Cite This Study

Distribution of cholesterol and apolipoprotein A-I and A-II in human high density lipoprotein subfractions separated by CsCl equilibrium gradient centrifugation: evidence for HDL subpopulations with differing A-I/A-II molar ratios.

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider