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January 1, 2001The Japanese Journal of PhysiologyOpen Access

Properties of a Ca2+-Activated Large Conductance K+ Channel with ATP Sensitivity in Human Renal Proximal Tubule Cells.

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Key result

In human renal proximal tubule epithelial cells, the activity of the Ca2+-activated large conductance K+ channel is directly inhibited by intracellular ATP independent of phosphorylation processes.

Population

Cultured human renal proximal tubule epithelial cells (RPTECs)

Design

Preclinical

Authors

JHJunko HiranoTeikyo University Chiba Medical CenterKNKazuyoshi NakamuraKisarazu HospitalMKManabu KubokawaIwate Medical University

Discussion

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Implication

Should not change practice; leaves open the in vivo role of ATP-sensitive BK channels in human renal tubules.

Structured PICO

P
Population
In vitro patch-clamp study of primary cultured human renal proximal tubule epithelial cells to investigate the properties of BK channels.
I
Intervention
Patch-clamp technique evaluating response to Ca2+, membrane potential, K+ channel blockers, and ATP
O
Outcome
BK channel activity and electrophysiological propertiessurrogate

This preclinical study characterizes a BK channel in human renal proximal tubule cells that is uniquely inhibited by intracellular ATP.

Limitations

  • The physiological role of the BK channel in RPTECs, such as participation in volume regulation, was not examined.
  • It is unknown whether this BK channel is present in the apical or basolateral membrane of human proximal tubule cells in situ.

Cite This Study

Hirano et al. (2001) studied Normal human kidney cells (RPTECs). Intracellular ATP and ATP analogues vs. Control bath solution without ATP was evaluated on Channel open probability (PO) / channel activity. In human renal proximal tubule epithelial cells, the activity of the Ca2+-activated large conductance K+ channel is directly inhibited by intracellular ATP independent of phosphorylation processes.

synapsesocial.com/papers/6a9d06a418679b9e3782d7bbhttps://doi.org/10.2170/jjphysiol.51.481
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