Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
January 1, 2001The Japanese Journal of PhysiologyOpen Access

An ATP-Regulated and pH-Sensitive Inwardly Rectifying K+ Channel in Cultured Human Proximal Tubule Cells.

View Full Paper
Ask AI
Bookmark
Share

Key result

ATP, cAMP/PKA, and intracellular pH regulate inwardly rectifying K+ channels in human proximal tubule cells.

Why the study?

Little is known about the properties and regulation of K(+) channels in human proximal tubule cells.

Population

Cultured human proximal tubule cells of normal kidney origin

Comparison

ATP, AMP-PNP, protein kinase inhibitors, cAMP analog, PKA-CS, and pH changes versus control conditions

Design

Patch-clamp electrophysiological study

Authors

KNKazuyoshi NakamuraJHJunko HiranoMKManabu Kubokawa

Discussion

Loading...

Member takes

Overview

No immediate clinical implications for tubular disorders; leaves open the channel's in vivo role in renal K+ homeostasis.

Structured PICO

P
Population
In vitro patch-clamp study of cultured human proximal tubule epithelial cells of normal kidney origin.
I
Intervention
Application of ATP, AMP-PNP, protein kinase inhibitors (K252a, KT5720), cAMP analog (8Br-cAMP), PKA-CS, and pH changes
C
Comparator
Control conditions (absence of agents or baseline)
O
Outcome
Properties and activity of the inwardly rectifying K(+) channel (conductance, ATP dependence, pH sensitivity)surrogate

This study characterizes an ATP-dependent, pH-sensitive inwardly rectifying K+ channel in human proximal tubule cells, regulated by cAMP/PKA.

Limitations

  • Precise morphological analyses were not performed to confirm basolateral origin
  • Direct evidence for the mechanisms of the pH effect was not provided

Cite This Study

Nakamura et al. (2001) studied Normal human kidney (in vitro). Intracellular ATP, cAMP/PKA, and pH alterations vs. Control conditions was evaluated on K+ channel activity (NPo). An inwardly rectifying K+ channel in cultured human proximal tubule cells was found to be ATP-dependent, regulated by cAMP/PKA-mediated phosphorylation, and sensitive to intracellular pH.

synapsesocial.com/papers/6aa9f2d252cffb64cd35db1dhttps://doi.org/10.2170/jjphysiol.51.523
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Properties of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule1998 · 38 citations
  2. 2Two K(+)‐selective conductances in single proximal tubule cells isolated from frog kidney are regulated by ATP.1997 · 15 citations
  3. 3Sensitivity of a renal K+ channel (ROMK2) to the inhibitory sulfonylurea compound glibenclamide is enhanced by coexpression with the ATP-binding cassette transporter cystic fibrosis transmembrane regulator.1996 · 208 citations
  4. 4Regulation of basolateral K channels in proximal tubule studied during continuous microperfusion1993 · 63 citations
  5. 5Cloning and characterization of multiple forms of the human kidney ROM-K potassium channel.1994 · 127 citations