Key result
An ELISA using antipeptide antibodies against HSV glycoprotein gB detected HSV antigen in 80% and 77% of confirmed HSE cases for peptides QLHDLRF and MKALYPLTT, respectively.
Why the study?
Can an ELISA protocol using antipeptide antibodies against HSV glycoprotein B accurately detect and quantify HSV antigen in the CSF of patients with suspected herpes simplex encephalitis?
Observational (n=180)
Can an ELISA protocol using antipeptide antibodies against HSV glycoprotein B accurately detect and quantify HSV antigen in the CSF of patients with suspected herpes simplex encephalitis?
An ELISA protocol using antipeptide antibodies against HSV glycoprotein B can detect and quantify HSV antigen in the CSF, offering a potential diagnostic tool for herpes simplex encephalitis.
Herpes simplex encephalitis (HSE) represents one of the most severe infectious diseases of the central nervous system (CNS). As effective antiviral drugs are available, an early, rapid, and reliable diagnosis has become important. The objective of this article was to develop a sensitive ELISA protocol for herpes simplex viruses (HSV) antigen detection and quantitation by assessing the usefulness of antipeptide antibodies against potential peptides of HSV glycoprotein B (gB). A total of 180 cerebrospinal fluid (CSF) samples of HSE and non-HSE patients were analyzed using a panel of antipeptide antibodies against synthetic peptides of HSV glycoprotein gB. The cases of confirmed and suspected HSE showed 80% and 51% positivity for antipeptide against synthetic peptide QLHDLRF and 77% and 53% positivity for antipeptide against synthetic peptide MKALYPLTT, respectively for the detection of HSV antigen in CSF. The concentration of HSV antigen was found to be higher in confirmed HSE as compared to suspected HSE group and the viral load correlated well with antigen concentration obtained using the two antipeptides in CSF of confirmed HSE group. This is the first article describing the use of antibodies obtained against synthetic peptides derived from HSV in diagnostics of HSE using patients' CSF samples.
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Bhullar et al. (2015) conducted an observational in Herpes simplex encephalitis (HSE) (n=180). ELISA using antipeptide antibodies against synthetic peptides of HSV glycoprotein gB vs. Non-HSE patients was evaluated on Detection of HSV antigen in CSF. An ELISA using antipeptide antibodies against HSV glycoprotein gB detected HSV antigen in 80% and 77% of confirmed HSE cases for peptides QLHDLRF and MKALYPLTT, respectively.
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