Key result
Expression of IRES specific IRNA in human hepatocarcinoma cells markedly inhibited HCV IRES-mediated translation and made cells refractory to poliovirus infection.
Why the study?
Does IRES specific IRNA inhibit HCV IRES mediated translation and poliovirus replication in human hepatocarcinoma cells?
Does IRES specific IRNA inhibit HCV IRES mediated translation and poliovirus replication in human hepatocarcinoma cells?
IRES specific IRNA inhibits HCV IRES mediated translation and poliovirus replication in vitro without detrimental effects on cell growth.
Does not support clinical use of IRNA; hypothesis-generating for IRES-targeted antivirals in HCV and poliovirus pending further studies.
AIM: To investigate the anti-virus infection activity of internal ribosome entry site (IRES) specific inhibitor RNA (IRNA). METHODS: IRNA eukaryotic vector pcRz-IRNA or mIRNA eukaryotic vector pcRz-mIRNA was transfected into human hepatocarcinoma cells (HHCC), then selected with neomycin G418 for 4 to 8 weeks, and then infected with polio virus vaccines line. The cytopethogenesis effect was investigated and the cell extract was collected. At last the polio virus titer of different cells was determined by plaque assay. RESULTS: Constructive expression of IRNA was not detrimental to cell growth. HCV IRES-mediated cap-independent translation was markedly inhibited in cells constructively expressing IRNA compared to control hepatoma cells. However, cap-dependent translation was not significantly affected in these cell line. Additionally, HHCC cells constitutively expressing IRNA became refractory to infection of polio virus. CONCLUSION: IRES specific IRNA can inhibit HCV IRES mediated translation and poliovirus replication.
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Xue-Song Liang (2003) studied HCV and poliovirus infection (in vitro). IRNA eukaryotic vector pcRz-IRNA vs. Control hepatoma cells / mIRNA eukaryotic vector pcRz-mIRNA was evaluated on HCV IRES-mediated cap-independent translation and poliovirus replication. Expression of IRES specific IRNA in human hepatocarcinoma cells markedly inhibited HCV IRES-mediated translation and made cells refractory to poliovirus infection.
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