Key result
In-vitro test systems using bovine neutrophils and platelets with HPLC were presented for evaluating the inhibition of 5-lipoxygenase and cyclo-oxygenase.
The study describes in-vitro test systems using bovine neutrophils and platelets coupled with HPLC to evaluate inhibitors of 5-lipoxygenase and cyclo-oxygenase.
Offers bovine neutrophil/platelet assays for dual 5-LOX/COX inhibitor screening; hypothesis-generating with no immediate clinical implications.
Inhibition of 5-lipoxygenase has been determined by monitoring the formation of leukotriene B4 and 5-hydroxyeicosatetraenoic acid in bovine polymorphonuclear leucocytes. For evaluating the inhibition of cyclo-oxygenase two different test systems are presented: the first uses 12-hydroxyheptadecatrienoic acid produced by bovine platelets as an indicator of the cyclo-oxygenase activity; the second test system monitors the prostaglandin E2 formation by bovine platelets. All arachidonic acid metabolites are quantified by reverse-phase HPLC with UV-detection.
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Dannhardt et al. (1992) studied this question. 5-lipoxygenase and cyclo-oxygenase inhibitors was evaluated on Inhibition of 5-lipoxygenase and cyclo-oxygenase (formation of LTB4, 5-HETE, 12-HHT, PGE2). In-vitro test systems using bovine neutrophils and platelets with HPLC were presented for evaluating the inhibition of 5-lipoxygenase and cyclo-oxygenase.
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