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The biological transformation of the bioactive dihydroisocoumarin, (-)-mellein, isolated from the marine-derived fungus Cladosporium sp., was studied. The preparative-scale culture of (-)-mellein with a marine isolate of a bacterium Stappia sp. resulted in the isolation of its oxidized metabolite, (3R,4S)-4-hydroxymellein. The stereostructure of the metabolite obtained was assigned on the basis of detailed physicochemical data analyses.
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