Key result
Continuous static mechanical pressure of 1 MPa for 2 hours reduced cell survival by approximately 21.93% and significantly increased creatine kinase, intracellular Ca2+, and malondialdehyde (all P < 0.05).
Why the study?
The study was conducted to establish a cellular-level mechanical injury model for human skeletal muscle cells and investigate changes in mechanical effect mechanisms following static loading injury.
This in vitro model provides an experimental platform to study the mechanisms and potential treatments for skeletal muscle injury caused by static loading pressure.
Establishes an in vitro platform for pressure-induced muscle injury research; leaves open clinical translation.
OBJECTIVE: To establish a cellular-level mechanical injury model for human skeletal muscle cells and investigate changes in the mechanical effect mechanism after such injuries. METHODS: The FX-5000™ Compression System was used to apply constant static mechanical pressure to human skeletal muscle cells. A factorial design analysis was conducted to discover the optimal injury model by evaluating the correlation between the amount of pressure, the duration of mechanical stimulation, and the number of days of observation. Skeletal muscle cell injury was evaluated by measuring cell metabolism, morphology, and calcium homeostasis. RESULTS: concentration, and malondialdehyde content, decreased superoxide dismutase, and caused cell swelling and severe cytoplasmic vacuolization (all P < 0.05). CONCLUSION: This model of mechanically-injured human skeletal muscle cells provides an experimental model for the clinically common skeletal muscle injury caused by static loading pressure. It may be used to study the mechanism of action of treatment methods for mechanically injured skeletal muscle.
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Yuan Yuan (2024) studied Skeletal muscle injury. Continuous static mechanical pressure vs. Normal control group (no pressure) was evaluated on Cell survival rate and metabolic/morphological markers of injury (p=<0.05). Continuous static mechanical pressure of 1 MPa for 2 hours reduced cell survival by approximately 21.93% and significantly increased creatine kinase, intracellular Ca2+, and malondialdehyde (all P < 0.05).
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