Key result
Recombinant human TNF suppressed lipoprotein lipase activity and stimulated intracellular lipolysis in 3T3-L1 adipocytes, mimicking the effects of crude cachectin.
Why the study?
Does recombinant human TNF suppress LPL activity and stimulate lipolysis in 3T3-L1 cells?
Population
3T3-L1 cells (adipocytes)
Comparison
Recombinant human TNF vs Crude cachectin preparation
Design
Preclinical
Authors
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TNF may mediate infection-related adipocyte catabolism; leaves open translation to human cachexia.
Does recombinant human TNF suppress LPL activity and stimulate lipolysis in 3T3-L1 cells?
Recombinant human TNF acts identically to cachectin in suppressing lipoprotein lipase activity and enhancing lipolysis in adipocytes, suggesting its role in infection-related catabolism.
Kawakami et al. (1987) studied this question. Recombinant human TNF vs. Crude cachectin / baseline was evaluated on Lipoprotein lipase (LPL) activity and intracellular lipolysis. Recombinant human TNF suppressed lipoprotein lipase activity and stimulated intracellular lipolysis in 3T3-L1 adipocytes, mimicking the effects of crude cachectin.