There is some difference of opinion among experimenters as to the significance of the length of time that sperm cells remain in a glycerolated extender prior to freezing. The basis of this study was a comparison of results of using 0.5-hour and 18-hour equilibration times on bovine semen in egg yolk-sodium citrate and skimmilk semen extenders. The results were measured in terms of the percentage of motile sperm after thawing as compared with the percentage observed prior to freezing. The study made possible a determination of the differences between individual bulls in relation to recovery percentage and the significance of the length of storage period in evaluating semen samples. Editor.
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O’Dell et al. (1956) studied this question.
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