Key result
Perfusion of isolated rat hearts with aerobic substrate-free or hypoxic medium decreased calcium levels in mitochondrial and microsomal fractions, highlighting the need for exogenous substrate and oxygen.
Why the study?
Does lack of substrate and oxygen alter intracellular calcium stores in isolated rat hearts?
Does lack of substrate and oxygen alter intracellular calcium stores in isolated rat hearts?
Exogenous substrate and oxygen are essential for maintaining intracellular calcium stores in the heart.
Rat heart findings suggest ischemia disrupts calcium stores; hypothesis-generating and should not yet alter clinical practice.
The levels of calcium in mitochondrial and microsomal fractions decreased in isolated rat hearts which failed to generate contractile force on perfusion with aerobic substratefree medium for 2 hr. Similar changes in the contents of calcium in the subcellular fractions of the hearts, in which contractility decreased by 80% of the control, were noted on perfusion for 30 min with hypoxic medium. These results reveal the importance of exogenous substrate and oxygen for the maintenance of the intracellular stores of the heart.
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Tomlinson et al. (1973) studied Failing hearts due to lack of substrate and oxygen. Perfusion with aerobic substrate-free medium or hypoxic medium vs. Control was evaluated on Levels of calcium in mitochondrial and microsomal fractions. Perfusion of isolated rat hearts with aerobic substrate-free or hypoxic medium decreased calcium levels in mitochondrial and microsomal fractions, highlighting the need for exogenous substrate and oxygen.
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