Key result
Copper chelate affinity chromatography achieved a 6.5 log reduction for enveloped Sindbis virus and a 4.0 log reduction for non-enveloped poliovirus during factor IX purification.
Population
Enveloped and non-enveloped viruses during the purification of a high-purity factor IX
Design
Preclinical
Authors
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May enhance viral clearance in factor IX production; leaves open translation to human plasma fractionation.
Copper chelate affinity chromatography effectively reduces both enveloped and non-enveloped viruses during factor IX purification through a combination of inactivation and physical removal.
Roberts et al. (1994) studied this question. Copper chelate affinity chromatography with acidic wash buffer was evaluated on Virus reduction. Copper chelate affinity chromatography achieved a 6.5 log reduction for enveloped Sindbis virus and a 4.0 log reduction for non-enveloped poliovirus during factor IX purification.
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