Key result
RNA aptamers specific to the RNA-dependent RNA polymerase of foot-and-mouth disease virus inhibited enzymatic activity in vitro with IC50 values <20 nM and Ki values of 18-75 nM.
Effect estimate: IC50 <20 nM, Ki 18-75 nM
RNA aptamers specific to the foot-and-mouth disease virus RNA-dependent RNA polymerase can inhibit its enzymatic activity in vitro.
Aptamer inhibition of FMDV RdRp supports antiviral development; leaves open in vivo efficacy and clinical translation.
Foot-and-mouth disease virus causes a highly contagious disease of agricultural livestock and is of enormous economic importance. Replication of the RNA genome of the virus, via negative strand intermediates, involves an RNA-dependent RNA polymerase (3Dpol). RNA aptamers specific to this enzyme have been selected and characterized. Some of these molecules inhibit enzymatic activity in vitro, with IC50 values of <20 nM and Ki values of 18-75 nM. Two of these show similarity, both with each other and with regions of the viral genome. Furthermore, truncated versions of one of the aptamers have been used to define the parts of the molecule responsible for its inhibitory activity.
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Ellingham et al. (2006) studied Foot-and-mouth disease virus. RNA aptamers specific to RNA-dependent RNA polymerase (3Dpol) was evaluated on Enzymatic activity inhibition in vitro (IC50 <20 nM, Ki 18-75 nM). RNA aptamers specific to the RNA-dependent RNA polymerase of foot-and-mouth disease virus inhibited enzymatic activity in vitro with IC50 values <20 nM and Ki values of 18-75 nM.
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