Key result
In cultured fibroblasts, myosin assembly correlated with high phosphorylation levels, and disassembly by trypsin or EGTA was preceded by a transient increase in intracellular calcium.
Population
Balb/c 3T3 cells and other cultured fibroblasts
Design
Preclinical
Authors
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Cautions against extrapolating fibroblast data to cardiac cells; leaves open calcium-myosin links in cardiovascular remodeling.
In cultured fibroblasts, changes in myosin assembly and phosphorylation are often correlated with transient changes in intracellular calcium concentration.
Rees et al. (1989) studied Cultured fibroblasts. Trypsin, EGTA, azide, and cAMP alteration was evaluated on Changes in cytoskeletal myosin distribution and cellular calcium concentration. In cultured fibroblasts, myosin assembly correlated with high phosphorylation levels, and disassembly by trypsin or EGTA was preceded by a transient increase in intracellular calcium.
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