Component Identification and Analysis of Vesicular Fluid From Swine Infected by Foot-and-Mouth Disease Virus

Figures

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  Figure 1. Venn diagram for serum protein identification. After preparation, the samples were lyophilized and incubated at 37°C for 16–18 h with 40 μl trypsin buffer. The hydrolysates were separated using capillary high-performance liquid chromatography and analyzed using Thermo Fisher mass spectrometry. MaxQuant 1.5.5.1 Searched the corresponding database, and finally obtained the results of protein identification and quantitative analysis.

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  Figure 2. Enrichment analysis of the vesicular fluid by Proteomics. (A) By gene ontology, (http://www.geneontology.org/) in the GO database, the enrichment analysis of biological processes (BP), cell components (CC), and molecular functions (MF) is significant. The abscissa indicates the enrichment to GO functional classification. The figure shows the enrichment analysis results of BP, CC, and MF. (B,C) Kyoto Encyclopedia of Genes and Genomes (KEGG): the ordinate represents the top 10 significantly enriched KEGG pathways and the abscissa represents the enrichment factor of each KEGG pathway (rich factor ≤ 1). Enrichment factor indicates the proportion of the number of differentially expressed proteins involved in a certain KEGG pathway among all identified proteins; the color of bubbles indicates the significance of the enriched KEGG pathway.

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  Figure 3. Correlation in protein abundance PPI using String. String (http://string-db.org/): the direct and indirect interactions between target proteins are found based on the information in the database.

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  Figure 4. Differential expression of cytokines in the vesicular fluid. After centrifuging the aspirated vesicular fluid, the supernatant and healthy pig serum were absorbed, diluted 10 times using the standard of ELISA kit, and the absorbance value was obtained at 450 nm in strict accordance with the manufacturer's instructions; then the cytokine content was calculated. (A,B) ELISA, determination of the expression of type I interferon; (C–F) ELISA, determination of the expression of inflammatory cytokines in the vesicular fluid; (G) ELISA, determination of the expression of IFN-γ. T-test was used to calculate the significant difference, which is marked as *P < 0.05, **P < 0.01, ***P < 0.001 in the figure.