Detection of Foot-and-Mouth Disease Virus in Swine Meat Juice

Figures

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  Figure 1. Detection of Foot-and-mouth disease virus (FMDV) in meat juice (MJ), serum (Ser), and oral swabs (OS) by rRT-PCR. Skeletal muscle (biceps femoris) was collected from animals experimentally infected with FMDV A22 IRQ 24/64 and MJ harvested after freeze-thaw cycles of skeletal muscle. Ribonucleic acid (RNA) was extracted from MJ, Ser, and OS collected at corresponding time points and tested by each rRT-PCR. The cut-off Ct value for the rRT-PCR is 40. Samples with Ct values less than 40 are considered positive for FMDV.

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  Figure 2. Crossing threshold (Ct) values for rRT-PCR for various tissue suspensions (submandibular lymph nodes, prescapular lymph nodes, popliteal lymph nodes, and tonsils) from FMDV A22 IRQ 24/64 infected animals. 10% tissue suspensions were prepared in PBS and clarified by centrifugation. RNA was extracted from tissue suspensions and tested by rRT-PCR. The cut-off Ct value for the rRT-PCR is 40. Samples with Ct values less than 40 are considered positive for FMDV.

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  Figure 3. Detection of FMDV in meat juice (MJ), serum (Ser), and oral swabs (OS) by rRT-PCR. Skeletal muscle (biceps femoris) was collected from animals experimentally infected with FMDV SAT2 ZIM 5/81 and meat Juice (MJ) harvested after freeze-thaw cycles of skeletal muscle. RNA was extracted from MJ, Ser, and OS collected at corresponding time points and tested by each rRT-PCR. Cut off Ct value for the rRT-PCR is 40. Samples with Ct values less than 40 are considered positive for FMDV.

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  Figure 4. Crossing threshold (Ct) values for rRT-PCR for various tissue suspensions (submandibular lymph nodes, prescapular lymph nodes, popliteal lymph nodes, and tonsils) from FMDV SAT2 ZIM 5/81 infected animals. 10% tissue suspensions were prepared in PBS and clarified by centrifugation. RNA was extracted from tissue suspensions and tested by rRT-PCR. Cut off Ct value for the rRT-PCR is 40. Samples with Ct values less than 40 are considered positive for FMDV.

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  Figure 5. Detection of FMDV serotype A antigen in meat juice by lateral flow immunoassay. Meat juice was harvested from pigs inoculated with FMDV A22 IRQ 24/64 and tested for FMDV serotype A antigen by lateral flow immunoassay. + = positive for FMDV antigen, DPI = days post-infection.

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  Figure 6. Detection of FMDV SAT 2 antigen in meat juice by lateral flow strip test. Meat juice was harvested from pigs inoculated with FMDV SAT2 ZIM 5/81 and tested for FMDV serotype SAT 2 antigen by lateral flow immunoassay. + = positive for FMDV antigen, DPI = days post-infection.