May 1, 1998Veterinary QuarterlyOpen Access

Detection of foot‐and‐mouth disease by reverse transcription polymerase chain reaction and virus isolation in contact sheep without clinical signs of foot‐and‐mouth disease

View Full Paper

Ask AI

Bookmark

Population

2 experimentally infected non-vaccinated sheep, 4 contact sheep, and 2 sentinel pigs

Design

Preclinical

Follow-up

at least one month

Authors

Discussion

Subclinical sheep may transmit FMDV; leaves open whether RT-PCR-ELISA enhances field surveillance in livestock.

Population

2 experimentally infected non-vaccinated sheep, 4 contact sheep, and 2 sentinel pigs

Intervention

Experimental infection with FMDV and contact exposure

Outcome

Presence of FMDV detected by RT-PCR and virus isolation in various secretion and tissue samplessurrogate

Subclinically infected sheep can harbor and transmit FMDV, which can be detected by sensitive RT-PCR-ELISA.

Callens et al. (1998) studied this question.

View Full Paper

Ask AI

Bookmark