Key result
Immunohistochemical studies using a novel antiserum specific for cleaved ANF showed no staining in unfixed frozen sections of rat atrial myocardium, suggesting pro-ANF processing is not intracardiocytic.
Population
Rabbits (for antiserum generation) and rat models (isolated perfused rat heart and rat atrial myocardium)
Design
Preclinical
Authors
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Questions intracardiocytic pro-ANF cleavage in rats; leaves open extracellular site and human relevance.
The lack of intracellular staining in rat atrial myocardium with a cleavage-specific antiserum suggests that the proteolytic processing of pro-ANF to its active hormonal form is not an intracardiocytic event.
Wildey et al. (1988) studied this question. anti-ANF-(99-105) antiserum was evaluated on Binding affinity and immunohistochemical staining of pro-ANF cleavage. Immunohistochemical studies using a novel antiserum specific for cleaved ANF showed no staining in unfixed frozen sections of rat atrial myocardium, suggesting pro-ANF processing is not intracardiocytic.
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