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May 1, 1988Journal of Clinical InvestigationOpen Access

Clearance and early hydrolysis of atrial natriuretic factor in vivo. Structural analysis of cleavage sites and design of an analogue that inhibits hormone cleavage.

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Population

Preclinical models: adult male Sprague-Dawley rats and mature female beagle dogs.

Comparison

Intravenous or intra-renal injection of… vs Native radiolabeled ANF.

Design

Preclinical

Follow-up

Up to 30 minutes post-injection

Authors

CCCindra CondraUnited States Military AcademyELE A LeidyMerck & Co., Inc., Rahway, NJ, USA (United States)PBPeter S. BuntingLancashire Teaching Hospitals NHS Foundation Trust

Discussion

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Implication

Supports preclinical ANF analogue optimization; leaves open translation to human cardiovascular therapy.

Structured PICO

P
Population
Preclinical models: adult male Sprague-Dawley rats and mature female beagle dogs.
I
Intervention
Intravenous or intra-renal injection of radiolabeled atrial natriuretic factor (125I-rANF) or a synthetic analogue containing aminoisobutyric acid at position 13 (125I-rAIB13(3-28)).
C
Comparator
Native radiolabeled ANF (for the analogue comparison).
O
Outcome
Clearance kinetics (half-life) and identification of metabolic cleavage fragments of ANF in vivo.surrogate

The identification of specific in vivo cleavage sites of ANF enables the design of synthetic analogues with enhanced stability against enzymatic degradation.

Limitations

  • Caution should be exercised in extrapolating from whole body radioautography studies to sites of ANF clearance which are physiologically relevant.

Cite This Study

Condra et al. (1988) studied this question.

synapsesocial.com/papers/6a76fb1e4fe2e6ffd8ec524dhttps://doi.org/10.1172/jci113462
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Degradation of atrial natriuretic factor in the rat1986 · 59 citations
  2. 2Biochemical Studies of Rat Atrial Natriuretic Factor1985 · 14 citations
  3. 3Specific membrane receptors for atrial natriuretic factor in renal and vascular tissues.1984 · 262 citations
  4. 4Identification of multiple binding sites for atrial natriuretic factor by affinity cross-linking in cultured endothelial cells.1986 · 228 citations
  5. 5Atrial natriuretic peptide: what is the excitement all about?1986 · 50 citations