Key result
A column chromatographic method for isolating CPK-MB and LDH 1,2 demonstrated sensitivities of 100% and 95%, and specificities of 96% and 86%, respectively, for diagnosing acute myocardial infarction.
Why the study?
Does a column chromatographic method for CPK-MB and LDH 1,2 isolation provide high sensitivity and specificity for diagnosing acute myocardial infarction?
Does a column chromatographic method for CPK-MB and LDH 1,2 isolation provide high sensitivity and specificity for diagnosing acute myocardial infarction?
A rapid column chromatographic method for isolating CPK-MB and LDH 1,2 provides high sensitivity and specificity for diagnosing acute myocardial infarction.
May support enzyme-based MI diagnosis in select settings; leaves open validation against modern troponin assays.
A column chromatographic method for the simultaneous single-eluate isolation of CPK-MB and LDH 1,2 has been evaluated. The method is rapid in that the entire isolation is completed in less than an hour and subsequent analysis can be performed using presently available spectrophotometric methods and reagents without the need for electrophoresis equipment. In addition, since the isoenzymes are directly isolated, quantitation of enzymatic activity can be performed with precision. This technic permits detection of CPK-MB and LDH 1,2 in samples with normal values of total CPK and LDH utilizing a routine laboratory spectrophotometer. The sensitivities for diagnosis of acute myocardial infarction are 95% for LDH 1,2 and 100% for CPK-MB. The specificities for diagnosis of acute myocardial infarction are 86% for LDH 1,2 and 96% for CPK-MB.
No takes yet. Share an insight, caveat, or question.
Lederer et al. (1976) studied Acute myocardial infarction. Column chromatographic method for isolation of CPK-MB and LDH 1,2 was evaluated on Sensitivity and specificity for diagnosis of acute myocardial infarction. A column chromatographic method for isolating CPK-MB and LDH 1,2 demonstrated sensitivities of 100% and 95%, and specificities of 96% and 86%, respectively, for diagnosing acute myocardial infarction.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: