January 1, 1974Clinical Chemistry

Separation of Tissue and Serum Creatine Kinase Isoenzymes by Ion-Exchange Column Chromatography

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Why the study?

Does anion-exchange column chromatography accurately separate CK isoenzymes and correlate with clinical myocardial infarction?

Population

71 patients with myocardial infarction and other diseases associated with elevated CK activity, and extracts…

Design

Other

Authors

DMDonald W. MercerUniversity of Pittsburgh

Discussion

Member takes

Implication

May support MB isoenzyme for MI diagnosis in select cases; leaves open validation in larger prospective studies.

Structured PICO

Does anion-exchange column chromatography accurately separate CK isoenzymes and correlate with clinical myocardial infarction?

Population

71 patients with myocardial infarction and other diseases associated with elevated CK activity, and extracts of CK-rich tissues (skeletal muscle, cardiac muscle, and brain)

Intervention

Anion-exchange column chromatography (DEAE-Sephadex A-50) for separating creatine kinase (CK) isoenzymes

Outcome

Separation of CK isoenzymes MM, MB, and BB and correlation of cardiac pattern (MB isoenzyme) with clinical documentation of myocardial infarctionsurrogate

The described anion-exchange column chromatography technique effectively separates CK isoenzymes, with the presence of the MB isoenzyme showing 100% correlation with clinical myocardial infarction.

Cite This Study

Donald W. Mercer (1974) studied this question.

synapsesocial.com/papers/6a703406f44fa9f079ddc551 https://doi.org/10.1093/clinchem/20.1.36

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Separation of Tissue and Serum Creatine Kinase Isoenzymes by Ion-Exchange Column Chromatography

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Separation of Tissue and Serum Creatine Kinase Isoenzymes by Ion-Exchange Column Chromatography

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider