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Most work on the excretion of urinary steroids has been concerned with the analysis and characterization of unconjugated compounds, although it has been known since urinary steroid analysis commenced, that such compounds are excreted as conjugates. Undoubtedly this has been due to the relative ease with which free steroids can be extracted and estimated after hydrolysis, and to the corresponding difficulties encountered in experiments dealing with steroid conjugates. Very few of the many steroid metabolites known to occur in urine have been isolated in the form of their conjugates, and the mode of conjugation of many compounds is known only by inference from hydrolytic procedures which lead to their appearance in free form. The object of the present work, of which the first part is here reported is to find suitable means of analysing steroids in the form of their conjugates. The work so far has been restricted to 17-ketosteroid (17-KS) conjugates.
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Kellie et al. (1956) studied this question.
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