What question did this study set out to answer?

Determine the in vivo oncogenic effects of coinjecting simian virus 40 and v-myc gene sequences into developing mouse embryos.

April 1, 1985Molecular and Cellular Biology

Analysis of a transgenic mouse containing simian virus 40 and v-myc sequences.

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Authors

JSJudy A. SmallNational Institutes of Health DBD. G. BlairAndrews University SSS D ShowalterNational Institute of Allergy and Infectious Diseases

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Implication

Transgenic animal study demonstrates simian virus 40 expression drives choroid plexus papilloma development, suggesting host-tissue factors suppress tumor formation in vivo.

Key Points

Determine the in vivo oncogenic effects of coinjecting simian virus 40 and v-myc gene sequences into developing mouse embryos.
Coinjected plasmids containing the simian virus 40 (SV40) genome linked to the mouse metallothionein I gene and the avian myelocytomatosis virus MC29 v-myc gene into mouse embryos.
Screened surviving progeny (N=13) for transgene integration and harvested tissue samples from a positive founder mouse (M13) following sacrifice at 13 weeks.
Established primary cell lines to analyze DNA integration, SV40 and v-myc mRNA transcription, T-antigen expression, and in vitro growth phenotypes.
One surviving mouse (M13) harbored both SV40 and v-myc DNA sequences and developed a cranial choroid plexus papilloma at 13 weeks.
Derived primary cell lines expressed SV40 mRNA and large T antigen, whereas v-myc mRNA remained undetectable across all tested lines.
Cultured cells rapidly displayed progressive transformation over time, marked by altered morphology, elevated growth rates, serum-free growth, and colony formation in soft agar.

Cite This Study

Small et al. (1985) studied this question.

synapsesocial.com/papers/6a9f6aeaff2e3aa83ec076cc https://doi.org/10.1128/mcb.5.4.642

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Implication

Transgenic animal study demonstrates simian virus 40 expression drives choroid plexus papilloma development, suggesting host-tissue factors suppress tumor formation in vivo.

Analysis of a transgenic mouse containing simian virus 40 and v-myc sequences.

Discussion

Member takes

Implication

Key Points

Cite This Study

Analysis of a transgenic mouse containing simian virus 40 and v-myc sequences.

Discussion

Member takes

Implication

Key Points

Cite This Study

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