Key result
Hepatitis A virus 14S pentamers and 70S empty capsids synthesized in Escherichia coli successfully self-assembled and induced antibodies capable of neutralizing the virus.
Antigenic hepatitis A virus structures can be successfully produced in Escherichia coli, which may have implications for vaccine development.
Hypothesis-generating for E. coli-based HAV vaccine antigens; human trials needed before any clinical consideration.
The synthesis of 14S pentamers and 70S empty capsids of hepatitis A virus (HAV) has been accomplished by expressing the viral genome for periods of time longer than 4 h in Escherichia coli. HAV pentamers (14S) self-assembled into capsids (70S) in vitro. The antibodies induced by these structures recognized and neutralized HAV.
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Sánchez et al. (2003) studied this question. Hepatitis A virus 14S pentamers and 70S empty capsids synthesized in Escherichia coli successfully self-assembled and induced antibodies capable of neutralizing the virus.
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