What is the clinical evidence from this study?

Primary outcome: Conformational and/or local interaction pattern changes in Factor VIIa.

October 26, 2014Journal of Biological ChemistryOpen Access

Sites Involved in Intra- and Interdomain Allostery Associated with the Activation of Factor VIIa Pinpointed by Hydrogen-Deuterium Exchange and Electron Transfer Dissociation Mass Spectrometry

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Key result

Hydrogen/deuterium exchange mass spectrometry revealed that the conformational allosteric activation signal of Factor VIIa extends to the EGF1 domain in the light chain.

Population

Factor VIIa (FVIIa) protein

Comparison

Activation induced by cofactor tissue factor or… vs Free, inactive form of FVIIa

Design

Preclinical

Authors

HSHongjian SongNankai University OOOle H. OlsenMC2 Therapeutics (Denmark)EPEgon PerssonOldham Council

Discussion

Member takes

Implication

Hypothesis-generating for allosteric Factor VIIa targeting; leaves open translation to human coagulation disorders.

Structured PICO

Population

Factor VIIa (FVIIa) protein

Exposure

Activation induced by cofactor tissue factor or a covalent active site inhibitor

Comparator

Free, inactive form of FVIIa

Outcome

Conformational changes and local interaction patterns of individual residues

This basic science study maps the allosteric activation network of Factor VIIa, revealing how conformational signals propagate across its domains.

Cite This Study

Song et al. (2014) studied this question. Hydrogen/deuterium exchange mass spectrometry revealed that the conformational allosteric activation signal of Factor VIIa extends to the EGF1 domain in the light chain.

synapsesocial.com/papers/6a9ff9054e3da7c164efd858 https://doi.org/10.1074/jbc.m114.614297

View Full Paper

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Sites Involved in Intra- and Interdomain Allostery Associated with the Activation of Factor VIIa Pinpointed by Hydrogen-Deuterium Exchange and Electron Transfer Dissociation Mass Spectrometry

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Sites Involved in Intra- and Interdomain Allostery Associated with the Activation of Factor VIIa Pinpointed by Hydrogen-Deuterium Exchange and Electron Transfer Dissociation Mass Spectrometry

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider