What question did this study set out to answer?

Develop and evaluate a single-tube solid-phase radioimmunoassay to measure plasma renin activity through enzymatic generation and quantification of angiotensin-I.

January 1, 1981Folia Endocrinologica JaponicaOpen Access

A Simple Solid Phase Radioimmunoassay for Plasma Renin Activity

Q: What is the clinical evidence from this study?

Study design: Other. Population: Healthy (assay validation) (n=66). Intervention: Solid phase radioimmunoassay (RIA) for plasma renin activity vs. Established solid phase radioimmunoassay kit (Clinical Assay Kit). Primary outcome: Correlation with established solid phase radioimmunoassay kit (r=0.996, p=<0.01).

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Key result

A newly developed solid phase radioimmunoassay for plasma renin activity, utilizing PMSF and EDTA, correlated closely with an established assay kit (r=0.996) and allowed measurement in a single test tube.

Why the study?

Does the new solid-phase RIA method accurately measure plasma renin activity compared to established kits?

Population

66 healthy adults on unrestricted salt diet, and 81 clinical cases.

Comparison

Solid-phase radioimmunoassay for plasma renin… vs GammaCoat [125I]-Plasma Renin Activity RIA Kit.

Design

Other

Authors

KIKazushige IINUMAJapan Radioisotope Association IIIsao IkedaMeiji University MTM TakaiKyoto University

Discussion

Member takes

Implication

May simplify PRA measurement; leaves open routine adoption pending independent clinical validation.

Key Points

Develop and evaluate a single-tube solid-phase radioimmunoassay to measure plasma renin activity through enzymatic generation and quantification of angiotensin-I.
Incubated 200 µL plasma with PMSF and EDTA at pH 6.0 or 7.4 at 37°C for 1 hour to generate angiotensin-I.
Quantified generated angiotensin-I via solid-phase radioimmunoassay using antibody-coated polystyrene beads and pepstatin A across a 3-hour incubation at room temperature.
Observed analytical recovery of added angiotensin-I at 102 ± 28.6% (1.0 ng/mL) and 105 ± 15.2% (4.0 ng/mL), with intra-assay CV of 2.5–10.3% and inter-assay CV of 7.0–13.7%.
Correlated closely with an established assay kit (r = 0.996), reporting normal reference values of 1.03 ± 0.78 ng/mL/hr in supine position (n = 66) and 1.33 ± 1.15 ng/mL/hr in upright position.

Structured PICO

Does the new solid-phase RIA method accurately measure plasma renin activity compared to established kits?

Population

66 healthy adults and several clinical cases were evaluated to validate a newly developed solid phase radioimmunoassay for measuring plasma renin activity.

Intervention

Solid-phase radioimmunoassay (RIA) for plasma renin activity (PRA) using PMSF, EDTA, and pepstatin A.

Comparator

GammaCoat [125I]-Plasma Renin Activity RIA Kit (established solid-phase RIA kit).

Outcome

Plasma renin activity (PRA) measurement correlation, precision, and recovery.surrogate

Main Result

Effect estimate: r=0.996

p-value: p=<0.01

The developed solid-phase radioimmunoassay provides a simple, reproducible, and accurate method for measuring plasma renin activity in a single test tube.

Cite This Study

IINUMA et al. (1981) studied Healthy (assay validation) (n=66). Solid phase radioimmunoassay (RIA) for plasma renin activity vs. Established solid phase radioimmunoassay kit (Clinical Assay Kit) was evaluated on Correlation with established solid phase radioimmunoassay kit (r=0.996, p=<0.01). A newly developed solid phase radioimmunoassay for plasma renin activity, utilizing PMSF and EDTA, correlated closely with an established assay kit (r=0.996) and allowed measurement in a single test tube.

synapsesocial.com/papers/6aa00bc8d6bf23a1213514dc https://doi.org/10.1507/endocrine1927.57.11_1645

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January 1, 1981Folia Endocrinologica JaponicaOpen Access

A Simple Solid Phase Radioimmunoassay for Plasma Renin Activity

View Full Paper

Ask AI

Bookmark

Key result

Why the study?

Does the new solid-phase RIA method accurately measure plasma renin activity compared to established kits?

Population

66 healthy adults on unrestricted salt diet, and 81 clinical cases.

Comparison

Solid-phase radioimmunoassay for plasma renin… vs GammaCoat [125I]-Plasma Renin Activity RIA Kit.

Design

Other

Authors

KIKazushige IINUMAJapan Radioisotope Association IIIsao IkedaMeiji University MTM TakaiKyoto University

Discussion

Member takes

Implication

May simplify PRA measurement; leaves open routine adoption pending independent clinical validation.

Key Points

Develop and evaluate a single-tube solid-phase radioimmunoassay to measure plasma renin activity through enzymatic generation and quantification of angiotensin-I.
Incubated 200 µL plasma with PMSF and EDTA at pH 6.0 or 7.4 at 37°C for 1 hour to generate angiotensin-I.
Quantified generated angiotensin-I via solid-phase radioimmunoassay using antibody-coated polystyrene beads and pepstatin A across a 3-hour incubation at room temperature.
Observed analytical recovery of added angiotensin-I at 102 ± 28.6% (1.0 ng/mL) and 105 ± 15.2% (4.0 ng/mL), with intra-assay CV of 2.5–10.3% and inter-assay CV of 7.0–13.7%.
Correlated closely with an established assay kit (r = 0.996), reporting normal reference values of 1.03 ± 0.78 ng/mL/hr in supine position (n = 66) and 1.33 ± 1.15 ng/mL/hr in upright position.

Structured PICO

Does the new solid-phase RIA method accurately measure plasma renin activity compared to established kits?

Population

66 healthy adults and several clinical cases were evaluated to validate a newly developed solid phase radioimmunoassay for measuring plasma renin activity.

Intervention

Solid-phase radioimmunoassay (RIA) for plasma renin activity (PRA) using PMSF, EDTA, and pepstatin A.

Comparator

GammaCoat [125I]-Plasma Renin Activity RIA Kit (established solid-phase RIA kit).

Outcome

Plasma renin activity (PRA) measurement correlation, precision, and recovery.surrogate

Main Result

Effect estimate: r=0.996

p-value: p=<0.01

The developed solid-phase radioimmunoassay provides a simple, reproducible, and accurate method for measuring plasma renin activity in a single test tube.

Cite This Study

synapsesocial.com/papers/6aa00bc8d6bf23a1213514dc https://doi.org/10.1507/endocrine1927.57.11_1645

Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

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