What is the clinical evidence from this study?

Study design: Other. Population: Border disease virus (BDV) infection (n=38). Intervention: Quantitative real-time RT-PCR vs. Conventional RT-PCR. Primary outcome: Detection of BDV RNA (Kappa 0.702).

November 5, 2009Virology JournalOpen Access

Detection and quantification of pestivirus in experimentally infected pregnant ewes and their progeny

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Key result

Quantitative real-time RT-PCR demonstrated a 45.6% complementary sensitivity over conventional RT-PCR for detecting Border disease virus RNA, showing potential to discriminate persistent infections.

Population

13 virus- and antibody-negative, artificially inseminated pregnant ewes and their progeny

Design

Preclinical

Follow-up

Until lambing

Authors

AHAna HurtadoInstituto de Óptica "Daza de Valdés"ISIsbene Sánchez FBFelix BastidaEuskadiko Parke Teknologikoa

Discussion

Member takes

Implication

qRT-PCR may aid BDV detection in sheep; leaves open clinical translation pending validation studies.

Structured PICO

Population

13 pregnant ewes experimentally infected with Border disease virus at different stages of gestation and their 25 offspring.

Exposure

Experimental infection with ovine pestivirus (BDV-4 genotype, strain 0502234) at 3 phases of pregnancy (days 108, 76, and 55 of gestation)

Outcome

Detection and quantification of viral RNA in blood and tissues using quantitative real-time RT-PCR (qRT-PCR) compared to conventional RT-PCRsurrogate

Main Result

Effect estimate: Kappa 0.702

Quantitative real-time RT-PCR is a fast and sensitive method for detecting BDV RNA that can help discriminate between persistent infections and transient viraemia in sheep.

Limitations

Small sample size
Definitive confirmation of persistent infection would have required a second analysis a couple of weeks apart
Reduced number of viruses used to test analytical specificity

Cite This Study

Hurtado et al. (2009) studied Border disease virus (BDV) infection (n=38). Quantitative real-time RT-PCR vs. Conventional RT-PCR was evaluated on Detection of BDV RNA (Kappa 0.702). Quantitative real-time RT-PCR demonstrated a 45.6% complementary sensitivity over conventional RT-PCR for detecting Border disease virus RNA, showing potential to discriminate persistent infections.

synapsesocial.com/papers/6aa033c193f2eb14ce69669b https://doi.org/10.1186/1743-422x-6-189

View Full Paper

Ask AI

Bookmark

Detection and quantification of pestivirus in experimentally infected pregnant ewes and their progeny

Discussion

Member takes

Implication

Structured PICO

Main Result

Limitations

Cite This Study

Detection and quantification of pestivirus in experimentally infected pregnant ewes and their progeny

Discussion

Member takes

Implication

Structured PICO

Main Result

Limitations

Cite This Study

Also Consider

Also Consider