Key result
RT-PCR detected BDV in 7.9% of fetuses, 50% of stillbirths, and 50% of lambs from commercial flocks, making it more suitable for stillbirths and lambs, while ELISA is recommended for autolyzed fetuses.
Why the study?
Does ELISA or RT-PCR better detect Border disease virus in fetuses, stillbirths, and newborn lambs?
Population
67 fetuses, 6 stillbirths, and 11 lambs from 25 commercial flocks with suspicion of BDV abortion, and 3…
Design
Preclinical
Authors
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May inform sample-type specific BDV testing in sheep; hypothesis-generating and requires prospective validation before routine use.
Observational (n=109)
Yes
Does ELISA or RT-PCR better detect Border disease virus in fetuses, stillbirths, and newborn lambs?
RT-PCR is more suitable for stillbirths and lambs with clinical symptoms to confirm BDV, whereas antigen ELISA is recommended for fetuses with advanced autolysis.
García‐Pérez et al. (2009) conducted an observational in Border disease virus (BDV) infection (n=109). RT-PCR and ELISA antigen detection was evaluated on Detection of Border disease virus (BDV). RT-PCR detected BDV in 7.9% of fetuses, 50% of stillbirths, and 50% of lambs from commercial flocks, making it more suitable for stillbirths and lambs, while ELISA is recommended for autolyzed fetuses.
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