Comparison of Staphylococcal Virulence Factors, Biofilm Formation and Antibiotic Susceptibility Test Among Staphylococcus aureus Strains Isolated from Various Clinical Specimens

Staphylococcus aureus isolates from clinical samples such as urine, pus, wound swab, blood and other body fluids were included in this study.Detection of Coagulase enzyme, Haemolysin production, Lecithinase enzyme, Staphylokinase activity, protease enzyme assay, Lipase enzyme, Biofilm production and Antibiotic susceptibility test were detected.In this study a number of 135 Staphylococcus aureus isolates were collected from various clinical samples.Isolates were phenotypically investigated for some virulence factors (Coagulase, Clumping factor, Hemolysis, Staphylokinase, Lipase, Protease, Lecithinase and Biofilm production).The antibiotic resistance and virulence factors were correlated statistically by chi-square test.It is evidenced that Gentamicin and Cloxacillin resistance is significant with the Protease activity.Cloxacillin, Ciprofloxacin and Erythromycin resistance is significant with Lipase activity.Cloxacillin resistance is significant with Lecithinase activity.Cloxacillin resistance is significant with Staphylokinase activity.Ciprofloxacin resistance is significant with Hemolysin activity.The antibiotic resistance and Biofilm formation were correlated statistically by chi-square test.It is evidenced that Ciprofloxacin resistance is significant with the Biofilm formation.It will be helpful for clinicians for better understanding about antibiotics resistance exhibited by the Staphylococcus species, also it will provide a better understanding about different types of virulence factors produced and it's correlation with antibiotic resistance.