Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand

Figures

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  Fig 1. Genotype natures of the 11 gene segments of three Thai DS-1-like G9P[8] strains, DBM2017-016, DBM2017-203, and DBM2018-291, compared with those of selected HuRVA strains with known genomic constellations. The three Thai DS-1-like G9P[8] strains (DBM2017-016, DBM2017-203, and DBM2018-291) are shown in red, while the DS-1-like G1P[8] strains and their derivatives that have been reported are shown in blue. Three co-circulating DS-1-like G2P[4] strains (DBM2017-003, DBM2017-015, and DBM2018-105) and two Wa-like G9P[8] strains (DBM2017-014 and DBM2018-111) are shown in green. Gray shading indicates the gene segments with genotypes identical to those of the three Thai DS-1-like G9P[8] strains. aThe gene segments that are most similar to those of strain DBM2017-016. bThe gene segments that are most similar to those of strain DBM2017-203. cThe gene segments that are most similar to those of strain DBM2018-291.

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  Fig 2. Phylogenetic trees constructed from the nucleotide sequences of the G9-VP7 (a), P[8]-VP4 (b), I2-VP6 (c), R2-VP1 (d), C2-VP2 (e), M2-VP3 (f), A2-NSP1 (g), N2-NSP2 (h), T2-NSP3 (i), E2-NSP4 (j), and H2-NSP5 (k) genes of strains DBM2017-016, DBM2017-203, and DBM2018-291, and representative RVA strains. In all the trees, the positions of the three Thai DS-1-like G9P[8] strains are shown in red, while those of other DS-1-like intergenogroup reassortant strains are shown in blue. Co-circulating strains, DBM2017-003, DBM2017-015, DBM2018-105, DBM2017-014, and DBM2018-111, are shown in green. Bootstrap values of <75% are not shown. Scale bars: 0.02 (b), 0.05 (h and i), 0.1 (a, c, d, e, g, and k), and 0.2 (f and j) substitutions per nucleotide.