October 1, 2008Journal of Cardiovascular Pharmacology

High Glucose Concentration Increases Macrophage Cholesterol Biosynthesis in Diabetes Through Activation of the Sterol Regulatory Element Binding Protein 1 (SREBP1): Inhibitory Effect of Insulin

Q: What is the clinical evidence from this study?

Study design: Other. Population: Diabetes and atherosclerosis. Intervention: Insulin vs. Untreated diabetic mice and high glucose without insulin. Primary outcome: Macrophage cholesterol biosynthesis.

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Key result

Insulin reduces macrophage cholesterol biosynthesis ~81% in diabetic mice by modulating SREBP-1 expression.

Why the study?

Does insulin reduce macrophage foam cell formation and cholesterol biosynthesis in diabetic models?

Population

Diabetic mice and in vitro glucose-enriched macrophages

Comparison

Insulin injection and insulin incubation vs Age-matched untreated diabetic mice and…

Design

Preclinical

Authors

MKMarielle KaplanTechnion – Israel Institute of Technology RKRachel Kerry THTony HayekUniversity Medical Center Rizk Hospital

Discussion

Member takes

Implication

Insulin's suppression of diabetic macrophage foam cell formation is hypothesis-generating; leaves open translation to human atheroprotection.

Structured PICO

Does insulin reduce macrophage foam cell formation and cholesterol biosynthesis in diabetic models?

Population

Diabetic mice and in vitro glucose-enriched macrophages used to evaluate the effect of insulin on cholesterol biosynthesis and foam cell formation.

Intervention

Insulin injection (in vivo) and insulin incubation (in vitro)

Comparator

Age-matched untreated diabetic mice (in vivo) and macrophages incubated with high glucose but without insulin (in vitro)

Outcome

Macrophage cholesterol biosynthesis, lipid peroxidation, and foam cell formation markers (Ox-LDL uptake, CD36 mRNA, HMG-CoA reductase expression, SREBP-1 maturation)surrogate

Insulin inhibits diabetes-mediated macrophage foam cell formation and cholesterol synthesis by modulating SREBP-1 expression and maturation, providing insights into atherogenesis in diabetes.

Cite This Study

Kaplan et al. (2008) studied Diabetes and atherosclerosis. Insulin vs. Untreated diabetic mice and high glucose without insulin was evaluated on Macrophage cholesterol biosynthesis. Insulin treatment of diabetic mice significantly reduced macrophage cholesterol biosynthesis by 81% compared to untreated diabetic mice by modulating SREBP-1 expression.

synapsesocial.com/papers/6aa14691b8a7879f1e7c570e https://doi.org/10.1097/fjc.0b013e3181879d98

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Implication

Insulin's suppression of diabetic macrophage foam cell formation is hypothesis-generating; leaves open translation to human atheroprotection.

High Glucose Concentration Increases Macrophage Cholesterol Biosynthesis in Diabetes Through Activation of the Sterol Regulatory Element Binding Protein 1 (SREBP1): Inhibitory Effect of Insulin

Discussion

Member takes

Implication

Structured PICO

Cite This Study

High Glucose Concentration Increases Macrophage Cholesterol Biosynthesis in Diabetes Through Activation of the Sterol Regulatory Element Binding Protein 1 (SREBP1): Inhibitory Effect of Insulin

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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